TY - JOUR
T1 - Synaptosomal (Ca2+-Mg2+)-ATPase activity modulation by cyclic AMP
AU - Rodriguez, Ricardo
AU - Toledo, Alfonso
AU - Sabriá, Josefa
AU - Rodriguez, José
AU - Blanco, Isaac
PY - 1989/10/1
Y1 - 1989/10/1
N2 - Dibutyryl cyclic AMP, in a concentration-dependent manner, increased synaptosomal (Ca2+Mg2+)-ATPase activity, but in synaptic plasma membranes lacked any effect. The maximal enzyme activity in synaptosomes was increased by 38%, leaving unaltered the extrasynaptosomal Ca2+ concentration necessary to reach it. In the presence of 5 μM cyclic AMP, cyclic AMPependent protein kinase increased (30%) maximal (Ca2+-Mg2+)-ATPase activity in synaptic plasma membranes, but the apparent affinity for Ca2+ was not modified. This effect was partially inhibited (60%) by a cyclic AMP-dependent protein kinase inhibitor. The data suggest that synaptosomal (Ca2+-Mg2+)-ATPase activity is modulated by a cyclic AMP-dependent phosphorylation reaction. © 1989.
AB - Dibutyryl cyclic AMP, in a concentration-dependent manner, increased synaptosomal (Ca2+Mg2+)-ATPase activity, but in synaptic plasma membranes lacked any effect. The maximal enzyme activity in synaptosomes was increased by 38%, leaving unaltered the extrasynaptosomal Ca2+ concentration necessary to reach it. In the presence of 5 μM cyclic AMP, cyclic AMPependent protein kinase increased (30%) maximal (Ca2+-Mg2+)-ATPase activity in synaptic plasma membranes, but the apparent affinity for Ca2+ was not modified. This effect was partially inhibited (60%) by a cyclic AMP-dependent protein kinase inhibitor. The data suggest that synaptosomal (Ca2+-Mg2+)-ATPase activity is modulated by a cyclic AMP-dependent phosphorylation reaction. © 1989.
U2 - 10.1016/0006-2952(89)90617-5
DO - 10.1016/0006-2952(89)90617-5
M3 - Article
SN - 0006-2952
VL - 38
SP - 3219
EP - 3222
JO - Biochemical Pharmacology
JF - Biochemical Pharmacology
ER -