TY - JOUR
T1 - Subtractive proteomic approach to the endometrial carcinoma invasion front
AU - Monge, Marta
AU - Ramon Y Cajal, Santiago
AU - Colome, Nuria
AU - Gil-Moreno, Antonio
AU - Perez-Benavente, Asummpcio
AU - Castellvi, Josep
AU - Canals, Francesc
AU - Garcia, Angel
AU - Colas, Eva
AU - Pedrola, Nuria
AU - Dolcet, Xavier
AU - Matias-Guiu, Xavier
AU - Lopez-Lopez, Rafael
AU - Reventos, Jaume
AU - Abal, Miguel
AU - Doll, Andreas
AU - Xercavins, Jordi
PY - 2009/10/19
Y1 - 2009/10/19
N2 - Tumor invasion defines the transition between tissue-restricted carcinomas, related to good outcome as optimal surgery becomes possible, and metastatic tumors associated with poor prognosis and a dramatic decrease in survival. In endometrial cancer, myometrial infiltration represents a determinant parameter highly valuable in prognosis. To date, the identification of proteins involved in endometrial carcinoma invasion has been essentially conducted by immunohistochemical methods, without a global perception on the invasive front. Laser microdissection presents nowadays limitations to the profound spatiotemporal regulation from both the tumor and the surrounding stroma occurring at the invasive front. In this work, we attempted an alternative proteomic approach to characterize specific components of the tumor invasive front or its reactive stroma, by comparing the invasive area of an endometrial carcinoma with the noninvasive superficial tumor area and normal tissue from the same patients. This strategy led us to identify proteins involved in cellular morphology, assembly and movement, differentially expressed at the invasive front, as well as pathways like cell-to-cell signaling and interaction and a modulated response to oxidative stress as events related to endometrial carcinoma invasion. In conclusion, we could identify new players of myometrial infiltration by applying a subtractive proteomic approach to the endometrial carcinoma invasion front. © 2009 American Chemical Society.
AB - Tumor invasion defines the transition between tissue-restricted carcinomas, related to good outcome as optimal surgery becomes possible, and metastatic tumors associated with poor prognosis and a dramatic decrease in survival. In endometrial cancer, myometrial infiltration represents a determinant parameter highly valuable in prognosis. To date, the identification of proteins involved in endometrial carcinoma invasion has been essentially conducted by immunohistochemical methods, without a global perception on the invasive front. Laser microdissection presents nowadays limitations to the profound spatiotemporal regulation from both the tumor and the surrounding stroma occurring at the invasive front. In this work, we attempted an alternative proteomic approach to characterize specific components of the tumor invasive front or its reactive stroma, by comparing the invasive area of an endometrial carcinoma with the noninvasive superficial tumor area and normal tissue from the same patients. This strategy led us to identify proteins involved in cellular morphology, assembly and movement, differentially expressed at the invasive front, as well as pathways like cell-to-cell signaling and interaction and a modulated response to oxidative stress as events related to endometrial carcinoma invasion. In conclusion, we could identify new players of myometrial infiltration by applying a subtractive proteomic approach to the endometrial carcinoma invasion front. © 2009 American Chemical Society.
KW - 2D-DIGE
KW - BLVRB
KW - Metastasis
KW - Invasion front
KW - Endometrial carcinoma
KW - Subtractive proteomics
KW - SOD1
UR - https://dialnet.unirioja.es/servlet/articulo?codigo=6020736
U2 - 10.1021/pr900390t
DO - 10.1021/pr900390t
M3 - Article
SN - 1535-3893
VL - 8
SP - 4676
EP - 4684
JO - Journal of Proteome Research
JF - Journal of Proteome Research
IS - 10
ER -