TY - JOUR
T1 - Studies on papain action in the synthesis of Gly-Phe in two-liquid-phase media
AU - Feliu, Josep Anton
AU - de Mas, Carles
AU - López-Santín, Josep
PY - 1995/1/1
Y1 - 1995/1/1
N2 - The synthesis of the protected dipeptide BocGly-PheOMe has been carried out in an aqueous-organic two-liquid-phase system. The reaction has been catalyzed by the protease papain using trichloroethylene as organic phase with an organic phase-aqueous phase volume ratio, Vorg Vaq, of 4. The results of the present article can be summarized as follows: a) A relationship between enzyme deactivation, interfacial area, and interfacial tension is stated. The addition of 0.4% Tween 80 to the reaction system stabilized papain activity for > 50 h, overcoming the problems related to the presence of the organic solvent and shear stress due to mixing; b) undesirable parallel reactions are identified; c) the peptide yield was increased by working with a fed-batch strategy. © 1995.
AB - The synthesis of the protected dipeptide BocGly-PheOMe has been carried out in an aqueous-organic two-liquid-phase system. The reaction has been catalyzed by the protease papain using trichloroethylene as organic phase with an organic phase-aqueous phase volume ratio, Vorg Vaq, of 4. The results of the present article can be summarized as follows: a) A relationship between enzyme deactivation, interfacial area, and interfacial tension is stated. The addition of 0.4% Tween 80 to the reaction system stabilized papain activity for > 50 h, overcoming the problems related to the presence of the organic solvent and shear stress due to mixing; b) undesirable parallel reactions are identified; c) the peptide yield was increased by working with a fed-batch strategy. © 1995.
KW - effect of interphase on enzymes
KW - enzyme stability
KW - Papain
KW - peptide synthesis
KW - two-liquid-phase system
U2 - 10.1016/0141-0229(94)00140-M
DO - 10.1016/0141-0229(94)00140-M
M3 - Article
SN - 0141-0229
VL - 17
SP - 882
EP - 887
JO - Enzyme and Microbial Technology
JF - Enzyme and Microbial Technology
IS - 10
ER -