Structure-guided redesign of d-fructose-6-phosphate aldolase from E. coli: Remarkable activity and selectivity towards acceptor substrates by two-point mutation

Mariana Gutierrez; Teodor Parella; Jesús Joglar; Jordi Bujons; Pere Clapés

doi:10.1039/c1cc11069a

Structure-guided redesign of d-fructose-6-phosphate aldolase from E. coli: Remarkable activity and selectivity towards acceptor substrates by two-point mutation

Mariana Gutierrez, Teodor Parella, Jesús Joglar, Jordi Bujons, Pere Clapés

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Structure-guided re-design of the acceptor binding site of d-fructose-6-phosphate aldolase from E. coli leads to the construction of FSA A129S/A165G double mutant with an activity between 5- to >900-fold higher than that of wild-type towards N-Cbz-aminoaldehyde derivatives. © 2011 The Royal Society of Chemistry.

Idioma original	Anglès
Pàgines (de-a)	5762-5764
Revista	Chemical Communications
Volum	47
Número	20
DOIs	https://doi.org/10.1039/c1cc11069a
Estat de la publicació	Publicada - 28 de maig 2011

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10.1039/c1cc11069a

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title = "Structure-guided redesign of d-fructose-6-phosphate aldolase from E. coli: Remarkable activity and selectivity towards acceptor substrates by two-point mutation",

abstract = "Structure-guided re-design of the acceptor binding site of d-fructose-6-phosphate aldolase from E. coli leads to the construction of FSA A129S/A165G double mutant with an activity between 5- to >900-fold higher than that of wild-type towards N-Cbz-aminoaldehyde derivatives. {\textcopyright} 2011 The Royal Society of Chemistry.",

author = "Mariana Gutierrez and Teodor Parella and Jes{\'u}s Joglar and Jordi Bujons and Pere Clap{\'e}s",

year = "2011",

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doi = "10.1039/c1cc11069a",

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volume = "47",

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Structure-guided redesign of d-fructose-6-phosphate aldolase from E. coli: Remarkable activity and selectivity towards acceptor substrates by two-point mutation. / Gutierrez, Mariana; Parella, Teodor; Joglar, Jesús et al.
In: Chemical Communications, Vol. 47, Núm. 20, 28.05.2011, pàg. 5762-5764.

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TY - JOUR

T1 - Structure-guided redesign of d-fructose-6-phosphate aldolase from E. coli: Remarkable activity and selectivity towards acceptor substrates by two-point mutation

AU - Gutierrez, Mariana

AU - Parella, Teodor

AU - Joglar, Jesús

AU - Bujons, Jordi

AU - Clapés, Pere

PY - 2011/5/28

Y1 - 2011/5/28

N2 - Structure-guided re-design of the acceptor binding site of d-fructose-6-phosphate aldolase from E. coli leads to the construction of FSA A129S/A165G double mutant with an activity between 5- to >900-fold higher than that of wild-type towards N-Cbz-aminoaldehyde derivatives. © 2011 The Royal Society of Chemistry.

AB - Structure-guided re-design of the acceptor binding site of d-fructose-6-phosphate aldolase from E. coli leads to the construction of FSA A129S/A165G double mutant with an activity between 5- to >900-fold higher than that of wild-type towards N-Cbz-aminoaldehyde derivatives. © 2011 The Royal Society of Chemistry.

UR - https://www.scopus.com/pages/publications/79955572384

U2 - 10.1039/c1cc11069a

DO - 10.1039/c1cc11069a

M3 - Article

SN - 1359-7345

VL - 47

SP - 5762

EP - 5764

JO - Chemical Communications

JF - Chemical Communications

IS - 20

ER -

Structure-guided redesign of d-fructose-6-phosphate aldolase from E. coli: Remarkable activity and selectivity towards acceptor substrates by two-point mutation

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