Specific processing of meiosis-related transcript is linked to final maturation in human oocytes

Sara Pietroforte, Montserrat Barragan Monasterio, Anna Ferrer-Vaquer, Manuel Irimia, Elena Ibáñez, Mina Popovic, Rita Vassena, Filippo Zambelli

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Resum

Human meiosis in oocytes entails an intricate regulation of the transcriptome to support late oocyte growth and early embryo development, both crucial to reproductive success. Currently, little is known about the co- and post-transcriptional mRNA processing mechanisms regulating the last meiotic phases, which contribute to transcriptome complexity and influence translation rates. We analyzed gene expression changes, splicing and pre-mRNA processing in an RNA sequencing set of 40 human oocytes at different meiotic maturation stages, matured both in vivo and in vitro. We found abundant untranslated region (UTR) processing, mostly at the 3' end, of meiosis-related genes between the germinal vesicle (GV) and metaphase II (MII) stages, supported by the differential expression of spliceosome and pre-mRNA processing related genes. Importantly, we found very few differences among GV oocytes across several durations of IVM, as long as they did not reach MII, suggesting an association of RNA processing and successful meiosis transit. Changes in protein isoforms are minor, although specific and consistent for genes involved in chromosome organization and spindle assembly. In conclusion, we reveal a dynamic transcript remodeling during human female meiosis, and show how pre-mRNA processing, specifically 3'UTR shortening, drives a selective translational regulation of transcripts necessary to reach final meiotic maturation.
Idioma originalEnglish
Número d’articlegaad021
Nombre de pàgines14
RevistaMolecular Human Reproduction
Volum29
Número7
DOIs
Estat de la publicacióPublicada - 1 de juny 2023

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