Single and competitive protein adsorption on polymeric surfaces

Thanga Bhuvanesh Vijaya Bhaskar; Toralf Roch; Oscar Romero; Nan Ma; Karl Kratz; Andreas Lendlein

doi:10.1002/pat.3639

Single and competitive protein adsorption on polymeric surfaces

Thanga Bhuvanesh Vijaya Bhaskar, Toralf Roch, Oscar Romero, Nan Ma, Karl Kratz, Andreas Lendlein^*

^*Autor corresponent d’aquest treball

Producció científica: Contribució a revista › Article › Recerca › Avaluat per experts

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Resum

La resposta biològica a les superfícies materials sovint es veu influïda per capes de proteïnes formades en la interfície biològica. Comprendre aquesta capa de proteïnes és d'una importància cabdal per als dispositius biomèdics i els cultius cel·lulars, que sovint requereixen un recobriment de proteïnes per a un creixement òptim de les cèl·lules. Es va desenvolupar un sistema d'inserció que s'ajusta exactament en plaques estàndards de cultiu de teixits i es pot fer servir per a investigacions biològiques sense la influència del material de cultiu cel·lular. Les insercions preparades amb poliestirè (PS), policarbonat (PC), poli(estirè-co-acrilonitril) (PSAN) i poli(imida d'òter) (PEI) mostren una rugositat superficial similar i humectabilitat de manera que només la química és variada. Prèviament, es va trobar que les respostes d'adhesió a cèl·lules mare eren diferents per a aquestes insercions, possiblement degut als seus perfils d'adsorció de proteïnes diferents. Aquest treball va investigar si els grups funcionals superficials d'aquestes insercions influeixen la seva capacitat d'unió a proteïnes. L'adsorció simple i competitiva de dues proteïnes sanguínies més abundants, immunoglobulina G (IgG) i albúmina sèrica humana (HSA), sobre aquests polímers es va investigar etiquetant ambdues proteïnes amb diferents tincions d'infraroig proper (IR). PEI va mostrar la major propensió a l'adsorció de proteïnes en una adsorció simple i competitiva d'IgG i HSA mentre que PS va exhibir la menor capacitat d'adsorció, mentre que PC i PSAN van mostrar un perfil d'adsorció de proteïnes intermediari. La inèrcia química del PS podria ser el motiu per a aquesta baixa capacitat d'unió a proteïnes i creixement cel·lular limitat. En conclusió, es va establir un nou mètode: per detectar eficientment l'adsorció de proteïnes sobre superfícies polimèriques i usant aquest mètode: la importància cabdal de la composició química dels substrats polimèrics en el seu perfil d'adsorció de proteïnes podria ser provat.

Idioma original	Anglès
Pàgines (de-a)	1387-1393
Nombre de pàgines	7
Revista	Polymers for Advanced Technologies
Volum	26
Número	12
DOIs	https://doi.org/10.1002/pat.3639
Estat de la publicació	Publicada - 1 de des. 2015

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title = "Single and competitive protein adsorption on polymeric surfaces",

abstract = "The biological response to material surfaces is often influenced by protein layers formed at the bio-interface. Understanding this protein layer is of paramount importance for biomedical materials and cell culture devices, which often require protein coating for optimal cell growth. An insert system fitting exactly into standard tissue culture plates was developed and can be used for biological investigations without the influence of the cell culture material. Inserts prepared from polystyrene (PS), polycarbonate (PC), poly(styrene-co-acrylonitrile) (PSAN) and poly(ether imide) (PEI) exhibit a similar surface roughness and wettability so that only the chemistry is varied. Previously, stem cell adhesion responses were found to be different for these inserts, possibly because of their different protein adsorption profiles. This work investigated if the surface functional groups of these inserts influence their protein binding ability. Single and competitive adsorption of two most abundant blood proteins, human serum albumin (HSA) and immunoglobulin G (IgG) on these polymers was investigated by labeling both proteins with different near-infrared (IR) dyes. PEI showed the highest protein adsorption propensity in single and competitive adsorption of IgG and HSA while PS exhibited the least adsorption capability, whereas PSAN and PC showed an intermediate protein adsorption profile. Chemical inertness of PS could be the reason for this low protein binding ability and limited cell growth. Conclusively, a novel method to efficiently detect protein adsorption on polymer surfaces was established, and using this method the high relevance of the chemical composition of polymeric substrates on their protein adsorption profile could be proven.",

keywords = "Biomaterials, Cell culture devices, IR labeled proteins, Polymeric substrate, Protein adsorption",

author = "\{Vijaya Bhaskar\}, \{Thanga Bhuvanesh\} and Toralf Roch and Oscar Romero and Nan Ma and Karl Kratz and Andreas Lendlein",

note = "Publisher Copyright: {\textcopyright} 2015 John Wiley \& Sons, Ltd.",

year = "2015",

month = dec,

day = "1",

doi = "10.1002/pat.3639",

language = "English",

volume = "26",

pages = "1387--1393",

journal = "Polymers for Advanced Technologies",

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publisher = "John Wiley and Sons Ltd",

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T1 - Single and competitive protein adsorption on polymeric surfaces

AU - Vijaya Bhaskar, Thanga Bhuvanesh

AU - Roch, Toralf

AU - Romero, Oscar

AU - Ma, Nan

AU - Kratz, Karl

AU - Lendlein, Andreas

PY - 2015/12/1

Y1 - 2015/12/1

N2 - The biological response to material surfaces is often influenced by protein layers formed at the bio-interface. Understanding this protein layer is of paramount importance for biomedical materials and cell culture devices, which often require protein coating for optimal cell growth. An insert system fitting exactly into standard tissue culture plates was developed and can be used for biological investigations without the influence of the cell culture material. Inserts prepared from polystyrene (PS), polycarbonate (PC), poly(styrene-co-acrylonitrile) (PSAN) and poly(ether imide) (PEI) exhibit a similar surface roughness and wettability so that only the chemistry is varied. Previously, stem cell adhesion responses were found to be different for these inserts, possibly because of their different protein adsorption profiles. This work investigated if the surface functional groups of these inserts influence their protein binding ability. Single and competitive adsorption of two most abundant blood proteins, human serum albumin (HSA) and immunoglobulin G (IgG) on these polymers was investigated by labeling both proteins with different near-infrared (IR) dyes. PEI showed the highest protein adsorption propensity in single and competitive adsorption of IgG and HSA while PS exhibited the least adsorption capability, whereas PSAN and PC showed an intermediate protein adsorption profile. Chemical inertness of PS could be the reason for this low protein binding ability and limited cell growth. Conclusively, a novel method to efficiently detect protein adsorption on polymer surfaces was established, and using this method the high relevance of the chemical composition of polymeric substrates on their protein adsorption profile could be proven.

AB - The biological response to material surfaces is often influenced by protein layers formed at the bio-interface. Understanding this protein layer is of paramount importance for biomedical materials and cell culture devices, which often require protein coating for optimal cell growth. An insert system fitting exactly into standard tissue culture plates was developed and can be used for biological investigations without the influence of the cell culture material. Inserts prepared from polystyrene (PS), polycarbonate (PC), poly(styrene-co-acrylonitrile) (PSAN) and poly(ether imide) (PEI) exhibit a similar surface roughness and wettability so that only the chemistry is varied. Previously, stem cell adhesion responses were found to be different for these inserts, possibly because of their different protein adsorption profiles. This work investigated if the surface functional groups of these inserts influence their protein binding ability. Single and competitive adsorption of two most abundant blood proteins, human serum albumin (HSA) and immunoglobulin G (IgG) on these polymers was investigated by labeling both proteins with different near-infrared (IR) dyes. PEI showed the highest protein adsorption propensity in single and competitive adsorption of IgG and HSA while PS exhibited the least adsorption capability, whereas PSAN and PC showed an intermediate protein adsorption profile. Chemical inertness of PS could be the reason for this low protein binding ability and limited cell growth. Conclusively, a novel method to efficiently detect protein adsorption on polymer surfaces was established, and using this method the high relevance of the chemical composition of polymeric substrates on their protein adsorption profile could be proven.

KW - Biomaterials

KW - Cell culture devices

KW - IR labeled proteins

KW - Polymeric substrate

KW - Protein adsorption

UR - https://www.scopus.com/pages/publications/84955215122

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JO - Polymers for Advanced Technologies

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IS - 12

ER -

Single and competitive protein adsorption on polymeric surfaces

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