TY - JOUR
T1 - Sex identification of wolf (Canis lupus) using non-invasive samples
AU - Sastre, Natalia
AU - Francino, Olga
AU - Lampreave, Gabriel
AU - Bologov, Vladimir V.
AU - López-Martín, José María
AU - Sánchez, Armand
AU - Ramírez, Oscar
PY - 2008/3/21
Y1 - 2008/3/21
N2 - We have developed new specific primers for sex determination from forensic samples of wolves (Canis lupus), such as hair, saliva, faecal, tooth and urine samples. In order to improve molecular sexing, we performed a multiplex semi-nested polymerase chain reaction (PCR) and several replicated amplifications per sample to avoid errors in low quantity DNA samples, such as allelic dropout and false alleles. The sex of individuals is automatically determined by capillary electrophoresis with a fluorescently labelled internal sex-specific primer from each pair. Our method yielded sex identification on 100% of invasive samples and 93% of forensic samples, being one of the highest success rates obtained from wild animals. © 2008 Springer Science+Business Media B.V.
AB - We have developed new specific primers for sex determination from forensic samples of wolves (Canis lupus), such as hair, saliva, faecal, tooth and urine samples. In order to improve molecular sexing, we performed a multiplex semi-nested polymerase chain reaction (PCR) and several replicated amplifications per sample to avoid errors in low quantity DNA samples, such as allelic dropout and false alleles. The sex of individuals is automatically determined by capillary electrophoresis with a fluorescently labelled internal sex-specific primer from each pair. Our method yielded sex identification on 100% of invasive samples and 93% of forensic samples, being one of the highest success rates obtained from wild animals. © 2008 Springer Science+Business Media B.V.
KW - Canis lupus
KW - Non-invasive samples
KW - Sex identification
KW - Sex-specific primers
KW - Wolf
U2 - 10.1007/s10592-008-9565-6
DO - 10.1007/s10592-008-9565-6
M3 - Article
SN - 1566-0621
VL - 10
SP - 555
EP - 558
JO - Conservation Genetics
JF - Conservation Genetics
ER -