TY - JOUR
T1 - Scalable fabrication of immunosensors based on carbon nanotube polymer composites
AU - Mendoza, Ernest
AU - Orozco, Jahir
AU - Jiménez-Jorquera, Cecilia
AU - González-Guerrero, Ana B.
AU - Calle, Ana
AU - Lechuga, Laura M.
AU - Fernández-Sánchez, César
PY - 2008/1/1
Y1 - 2008/1/1
N2 - In this work we present the fabrication and characterization of immunosensors based on polystyrene (PS)-multiwalled carbon nanotube (MWCNT) composites. The electrochemical properties of the sensors have been investigated and show that the surface area is increased upon addition of the MWCNT-PS layer. Furthermore, a plasma activation process is used to partially remove the PS and expose the MWCNTs. This results in a huge increase in the electrochemical area and opens up the possibility of binding biomolecules to the MWCNT wall. The MWCNTs have been functionalized covalently with a model antibody (rabbit IgG). The biosensors have been tested using amperometric techniques and show detection limits comparable to standard techniques such as ELISA. © IOP Publishing Ltd.
AB - In this work we present the fabrication and characterization of immunosensors based on polystyrene (PS)-multiwalled carbon nanotube (MWCNT) composites. The electrochemical properties of the sensors have been investigated and show that the surface area is increased upon addition of the MWCNT-PS layer. Furthermore, a plasma activation process is used to partially remove the PS and expose the MWCNTs. This results in a huge increase in the electrochemical area and opens up the possibility of binding biomolecules to the MWCNT wall. The MWCNTs have been functionalized covalently with a model antibody (rabbit IgG). The biosensors have been tested using amperometric techniques and show detection limits comparable to standard techniques such as ELISA. © IOP Publishing Ltd.
U2 - 10.1088/0957-4484/19/7/075102
DO - 10.1088/0957-4484/19/7/075102
M3 - Article
SN - 0957-4484
VL - 19
JO - Nanotechnology
JF - Nanotechnology
IS - 7
M1 - 075102
ER -