Rapid quantification of riboflavin in milk by front-face fluorescence spectroscopy: A preliminary study

Ulises Alvarado, Anna Zamora, Jinfang Liu, Jordi Saldo, Manuel Castillo*

*Autor corresponent d’aquest treball

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Resum

The front-face fluorescence spectroscopy technique was used to establish a rapid prediction model of riboflavin concentration in milk without prior sample preparation. The prediction model developed was then compared with two conventional high performance liquid chromatography (HPLC)-based quantification methods. The method of standard addition allowed detecting a linear correlation between fluorescence intensity and riboflavin concentration in 12% (w/w) reconstituted low-heat milk powder. Validation of the model yielded an R2 of 0.99 with a standard error of prediction of 0.13 mg/L. The results suggest a potential use of front-face fluorescence spectroscopy as a simple method for off- and in-line determinations of riboflavin in milk.

Idioma originalAmerican English
Número d’article6
Nombre de pàgines6
RevistaFoods
Volum9
Número1
DOIs
Estat de la publicacióPublicada - de gen. 2020

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