TY - GEN
T1 - Rapid detection of viable bacteria in whole blood for early sepsis diagnosis and susceptibility testing
AU - Iyengar, Sharath Narayana
AU - Dietvorst, Jiri
AU - Ferrer-Vilanova, Amparo
AU - Guirado, Gonzalo
AU - Muñoz-Berbel, Xavier
AU - Russom, Aman
N1 - Publisher Copyright:
© 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.
PY - 2021
Y1 - 2021
N2 - Sepsis is a serious medical condition characterized by a whole-body inflammatory state caused by infection. Here, we present a sepsis method for rapid detection of bacteria from whole blood in less than 5h, combining selective blood cell lysis and a sensitive colorimetric based detection method. Selective cell lysis buffer allows selective rupture of blood cells (5 min), while maintaining bacteria 100% viable. Viable bacteria metabolically reduce iron (III) complexes, initiating a photo-catalytic cascade toward Prussian Blue formation visible to the naked eye. The method is finally validated for antibiotic susceptibility testing.
AB - Sepsis is a serious medical condition characterized by a whole-body inflammatory state caused by infection. Here, we present a sepsis method for rapid detection of bacteria from whole blood in less than 5h, combining selective blood cell lysis and a sensitive colorimetric based detection method. Selective cell lysis buffer allows selective rupture of blood cells (5 min), while maintaining bacteria 100% viable. Viable bacteria metabolically reduce iron (III) complexes, initiating a photo-catalytic cascade toward Prussian Blue formation visible to the naked eye. The method is finally validated for antibiotic susceptibility testing.
KW - bacteria
KW - blood
KW - colorimetric
KW - E.coli
KW - Prussian blue
KW - Selective cell lysis
KW - Sepsis
UR - http://www.scopus.com/inward/record.url?scp=85136914448&partnerID=8YFLogxK
M3 - Other contribution
AN - SCOPUS:85136914448
T3 - MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
ER -