Rapid detection of viable bacteria in whole blood for early sepsis diagnosis and susceptibility testing

Sharath Narayana Iyengar, Jiri Dietvorst, Amparo Ferrer-Vilanova, Gonzalo Guirado, Xavier Muñoz-Berbel, Aman Russom*

*Autor corresponent d’aquest treball

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Resum

Sepsis is a serious medical condition characterized by a whole-body inflammatory state caused by infection. Here, we present a sepsis method for rapid detection of bacteria from whole blood in less than 5h, combining selective blood cell lysis and a sensitive colorimetric based detection method. Selective cell lysis buffer allows selective rupture of blood cells (5 min), while maintaining bacteria 100% viable. Viable bacteria metabolically reduce iron (III) complexes, initiating a photo-catalytic cascade toward Prussian Blue formation visible to the naked eye. The method is finally validated for antibiotic susceptibility testing.

Idioma originalAnglès
Nombre de pàgines2
ISBN (electrònic)9781733419031
Estat de la publicacióPublicada - 2021

Sèrie de publicacions

NomMicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences

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