Quantitative modeling of inducer transport in fed-batch cultures of Escherichia coli

Daniel Calleja; Alfred Fernández-Castañé; Martina Pasini; Carles de Mas; Josep López-Santín

doi:10.1016/j.bej.2014.08.017

Quantitative modeling of inducer transport in fed-batch cultures of Escherichia coli

Daniel Calleja, Alfred Fernández-Castañé, Martina Pasini, Carles de Mas, Josep López-Santín

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© 2014 Elsevier B.V. All rights reserved. An unsteady, unstructured, unsegregated and based on first principles mathematical model has been proposed to describe IPTG (isopropyl-β-. d-tiogalactopiranoside) transport in induced fed-batch cultures of E. coli M15 δ. glyA [pQEαβrham] [pREP4] producing rhamnulose 1-phosphate aldolase (RhuA). The model predicts extracellular and intracellular IPTG concentration. Experimental extracellular IPTG concentrations under different operational conditions were obtained by HPLC-MS analysis. These experimental data were used to fit the parameters of the model. The model was also able to predict the experimental behavior of two different E. coli strains producing fuculose 1-phosphate aldolase (FucA). IPTG transport to cells was the contribution of three processes: a diffusion process, and two active processes (one non-specific and another specific).

Idioma original	English
Pàgines (de-a)	210-219
Revista	Biochemical Engineering Journal
Volum	91
DOIs	https://doi.org/10.1016/j.bej.2014.08.017
Estat de la publicació	Publicada - 5 d’oct. 2014

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10.1016/j.bej.2014.08.017

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@article{2675c1f364574bc5b04f3b18dbda7897,

title = "Quantitative modeling of inducer transport in fed-batch cultures of Escherichia coli",

abstract = "{\textcopyright} 2014 Elsevier B.V. All rights reserved. An unsteady, unstructured, unsegregated and based on first principles mathematical model has been proposed to describe IPTG (isopropyl-β-. d-tiogalactopiranoside) transport in induced fed-batch cultures of E. coli M15 δ. glyA [pQEαβrham] [pREP4] producing rhamnulose 1-phosphate aldolase (RhuA). The model predicts extracellular and intracellular IPTG concentration. Experimental extracellular IPTG concentrations under different operational conditions were obtained by HPLC-MS analysis. These experimental data were used to fit the parameters of the model. The model was also able to predict the experimental behavior of two different E. coli strains producing fuculose 1-phosphate aldolase (FucA). IPTG transport to cells was the contribution of three processes: a diffusion process, and two active processes (one non-specific and another specific).",

keywords = "E. coli, IPTG, Inducer transport, Lac permeases, Mathematical model",

author = "Daniel Calleja and Alfred Fern{\'a}ndez-Casta{\~n}{\'e} and Martina Pasini and {de Mas}, Carles and Josep L{\'o}pez-Sant{\'i}n",

year = "2014",

month = oct,

day = "5",

doi = "10.1016/j.bej.2014.08.017",

language = "English",

volume = "91",

pages = "210--219",

journal = "Biochemical Engineering Journal",

issn = "1369-703X",

publisher = "Elsevier",

}

TY - JOUR

T1 - Quantitative modeling of inducer transport in fed-batch cultures of Escherichia coli

AU - Calleja, Daniel

AU - Fernández-Castañé, Alfred

AU - Pasini, Martina

AU - de Mas, Carles

AU - López-Santín, Josep

PY - 2014/10/5

Y1 - 2014/10/5

N2 - © 2014 Elsevier B.V. All rights reserved. An unsteady, unstructured, unsegregated and based on first principles mathematical model has been proposed to describe IPTG (isopropyl-β-. d-tiogalactopiranoside) transport in induced fed-batch cultures of E. coli M15 δ. glyA [pQEαβrham] [pREP4] producing rhamnulose 1-phosphate aldolase (RhuA). The model predicts extracellular and intracellular IPTG concentration. Experimental extracellular IPTG concentrations under different operational conditions were obtained by HPLC-MS analysis. These experimental data were used to fit the parameters of the model. The model was also able to predict the experimental behavior of two different E. coli strains producing fuculose 1-phosphate aldolase (FucA). IPTG transport to cells was the contribution of three processes: a diffusion process, and two active processes (one non-specific and another specific).

AB - © 2014 Elsevier B.V. All rights reserved. An unsteady, unstructured, unsegregated and based on first principles mathematical model has been proposed to describe IPTG (isopropyl-β-. d-tiogalactopiranoside) transport in induced fed-batch cultures of E. coli M15 δ. glyA [pQEαβrham] [pREP4] producing rhamnulose 1-phosphate aldolase (RhuA). The model predicts extracellular and intracellular IPTG concentration. Experimental extracellular IPTG concentrations under different operational conditions were obtained by HPLC-MS analysis. These experimental data were used to fit the parameters of the model. The model was also able to predict the experimental behavior of two different E. coli strains producing fuculose 1-phosphate aldolase (FucA). IPTG transport to cells was the contribution of three processes: a diffusion process, and two active processes (one non-specific and another specific).

KW - E. coli

KW - IPTG

KW - Inducer transport

KW - Lac permeases

KW - Mathematical model

U2 - 10.1016/j.bej.2014.08.017

DO - 10.1016/j.bej.2014.08.017

M3 - Article

SN - 1369-703X

VL - 91

SP - 210

EP - 219

JO - Biochemical Engineering Journal

JF - Biochemical Engineering Journal

ER -

Quantitative modeling of inducer transport in fed-batch cultures of Escherichia coli

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