TY - JOUR
T1 - Quantification of amphetamine plasma concentrations by gas chromatography coupled to mass spectrometry
AU - Pizarro, Nieves
AU - Ortuño, Jordi
AU - Segura, Jordi
AU - Farré, Magí
AU - Mas, Marta
AU - Camí, Jordi
AU - De La Torre, Rafael
PY - 1999/12/1
Y1 - 1999/12/1
N2 - We developed a fast and sensitive method for identification and quantification of plasma concentrations of amphetamine using gas chromatography with mass spectrometry detection (GC-MS). Amphetamine-d8 served as internal standard. The method involves a single extraction procedure and an easy treatment of the samples that allowed no losses during the evaporation process. Derivatisation of amphetamine with N-methyl- bis(trifluoroacetamide), a potent acylating agent, provides many advantages to the method compared with common derivatisation reactions usually used for amphetamines. The limits of detection and quantification following this method were 0.43 and 1.42 ng/ml, respectively. The assay has been successfully employed in the quantification of amphetamine in plasma samples from healthy volunteers at four different doses.
AB - We developed a fast and sensitive method for identification and quantification of plasma concentrations of amphetamine using gas chromatography with mass spectrometry detection (GC-MS). Amphetamine-d8 served as internal standard. The method involves a single extraction procedure and an easy treatment of the samples that allowed no losses during the evaporation process. Derivatisation of amphetamine with N-methyl- bis(trifluoroacetamide), a potent acylating agent, provides many advantages to the method compared with common derivatisation reactions usually used for amphetamines. The limits of detection and quantification following this method were 0.43 and 1.42 ng/ml, respectively. The assay has been successfully employed in the quantification of amphetamine in plasma samples from healthy volunteers at four different doses.
KW - Amphetamine
KW - Gas Chromatography-Mass Spectrometry
KW - Pharmacokinetics
U2 - 10.1016/S0731-7085(99)00200-9
DO - 10.1016/S0731-7085(99)00200-9
M3 - Article
SN - 0731-7085
VL - 21
SP - 739
EP - 747
JO - Journal of Pharmaceutical and Biomedical Analysis
JF - Journal of Pharmaceutical and Biomedical Analysis
IS - 4
ER -