@inbook{7f78deca51c04e50b1d7a92ef253fb37,
title = "Protocols for Rational Design of Protein Solubility and Aggregation Properties Using Aggrescan3D Standalone",
abstract = "Protein aggregation is a major hurdle in the development and manufacturing of protein-based therapeutics. Development of aggregation-resistant and stable protein variants can be guided by rational redesign using computational tools. Here, we describe the architecture and functionalities of the Aggrescan3D (A3D) standalone package for the rational design of protein solubility and aggregation properties based on three-dimensional protein structures. We present the case studies of the three therapeutic proteins, including antibodies, exploring the practical use of the A3D standalone tool. The case studies demonstrate that protein solubility can be easily improved by the A3D prediction of non-destabilizing amino acid mutations at the protein surfaces.",
keywords = "Aggregation prediction, Aggrescan3D, Computational modeling, Protein aggregation, Protein design, Structure-based design",
author = "Aleksander Kuriata and Badaczewska-Dawid, \{Aleksandra E.\} and Jordi Pujols and Salvador Ventura and Sebastian Kmiecik",
note = "Funding Information: S.K. acknowledges support from the National Science Centre of Poland, grant MAESTRO no. 2014/14/A/ST6/00088. S.V. acknowledges support from the Spanish Ministry of Economy and Competitiveness no. BIO2016-78310-R and ICREA, ICREA-Academia 2015. J.P. acknowledges support from the Spanish Ministry of Science and Innovation via a doctoral grant (FPU14/ 07161). A.E.B-D. acknowledges financial support by Roy J. Carver Charitable Trust through Iowa State University Bioscience Innovation Postdoctoral Fellowship. Publisher Copyright: {\textcopyright} 2022, Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2022",
doi = "10.1007/978-1-0716-1546-1\_2",
language = "English",
series = "Methods in Molecular Biology",
pages = "17--40",
booktitle = "Methods in Molecular Biology",
}