Proteomic study via a non-gel based approach of meningococcal outer membrane vesicle vaccine obtained from strain CU385: a road map for discovering new antigens

Jeovanis Gil; L Zaro H Betancourt; Gretel Sardiñas; Daniel Yero; Olivia Niebla; Maité Delgado; Darien García; Rolando Pajón; Aniel Sánchez; Luis J González; Gabriel Padrón; Concepción Campa; Franklin Sotolongo; Ramón Barberó; Gerardo Guillén; Luis Herrera; Vladimir Besada

doi:10.4161/hv.5.5.7367

Proteomic study via a non-gel based approach of meningococcal outer membrane vesicle vaccine obtained from strain CU385: a road map for discovering new antigens

Jeovanis Gil, L Zaro H Betancourt, Gretel Sardiñas, Daniel Yero, Olivia Niebla, Maité Delgado, Darien García, Rolando Pajón, Aniel Sánchez, Luis J González, Gabriel Padrón, Concepción Campa, Franklin Sotolongo, Ramón Barberó, Gerardo Guillén, Luis Herrera, Vladimir Besada

Center for Genetic Engineering and Biotechnology

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This work presents the results from a study of the protein composition of outer membrane vesicles from VA-MENGOC-BC (Finlay Institute, Cuba), an available vaccine against serogroup B Neisseria meningitidis. Proteins were identified by means of SCAPE, a 2DE-free method for proteome studies. More than one hundred proteins were detected by tandem liquid chromatographymass spectrometry analysis of fractions enriched in peptides devoid of histidine or arginine residues, providing a detailed description of the vaccine. A bioinformatic analysis of the identified components resulted in the identification of 31 outer membrane proteins and three conserved hypothetical proteins, allowing the cloning, expression, purification and immunological study of two of them (NMB0088 and NMB1796) as new antigens.

Idioma original	Anglès nord-americà
Pàgines (de-a)	347-56
Nombre de pàgines	10
Revista	Human Vaccines
Volum	5
Número	5
DOIs	https://doi.org/10.4161/hv.5.5.7367
Estat de la publicació	Publicada - de maig 2009

SDG de les Nacions Unides

Aquest resultat contribueix als següents objectius de desenvolupament sostenible.

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10.4161/hv.5.5.7367

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Gil, J., Betancourt, L. Z. H., Sardiñas, G., Yero, D., Niebla, O., Delgado, M., García, D., Pajón, R., Sánchez, A., González, L. J., Padrón, G., Campa, C., Sotolongo, F., Barberó, R., Guillén, G., Herrera, L., & Besada, V. (2009). Proteomic study via a non-gel based approach of meningococcal outer membrane vesicle vaccine obtained from strain CU385: a road map for discovering new antigens. Human Vaccines, 5(5), 347-56. https://doi.org/10.4161/hv.5.5.7367

@article{fac0dae0ce1e45bdbb7914507e9c035a,

title = "Proteomic study via a non-gel based approach of meningococcal outer membrane vesicle vaccine obtained from strain CU385: a road map for discovering new antigens",

abstract = "This work presents the results from a study of the protein composition of outer membrane vesicles from VA-MENGOC-BC (Finlay Institute, Cuba), an available vaccine against serogroup B Neisseria meningitidis. Proteins were identified by means of SCAPE, a 2DE-free method for proteome studies. More than one hundred proteins were detected by tandem liquid chromatographymass spectrometry analysis of fractions enriched in peptides devoid of histidine or arginine residues, providing a detailed description of the vaccine. A bioinformatic analysis of the identified components resulted in the identification of 31 outer membrane proteins and three conserved hypothetical proteins, allowing the cloning, expression, purification and immunological study of two of them (NMB0088 and NMB1796) as new antigens.",

keywords = "Antigens, Bacterial/analysis, Bacterial Proteins/analysis, Chromatography, Liquid/methods, Cuba, Humans, Meningococcal Vaccines/chemistry, Neisseria meningitidis, Serogroup B/chemistry, Proteome/analysis, Secretory Vesicles/chemistry, Tandem Mass Spectrometry/methods",

author = "Jeovanis Gil and Betancourt, {L Zaro H} and Gretel Sardi{\~n}as and Daniel Yero and Olivia Niebla and Mait{\'e} Delgado and Darien Garc{\'i}a and Rolando Paj{\'o}n and Aniel S{\'a}nchez and Gonz{\'a}lez, {Luis J} and Gabriel Padr{\'o}n and Concepci{\'o}n Campa and Franklin Sotolongo and Ram{\'o}n Barber{\'o} and Gerardo Guill{\'e}n and Luis Herrera and Vladimir Besada",

year = "2009",

month = may,

doi = "10.4161/hv.5.5.7367",

language = "Ingl{\'e}s estadounidense",

volume = "5",

pages = "347--56",

journal = "Human Vaccines",

issn = "1554-8600",

number = "5",

}

Gil, J, Betancourt, LZH, Sardiñas, G, Yero, D, Niebla, O, Delgado, M, García, D, Pajón, R, Sánchez, A, González, LJ, Padrón, G, Campa, C, Sotolongo, F, Barberó, R, Guillén, G, Herrera, L & Besada, V 2009, 'Proteomic study via a non-gel based approach of meningococcal outer membrane vesicle vaccine obtained from strain CU385: a road map for discovering new antigens', Human Vaccines, vol. 5, núm. 5, pàg. 347-56. https://doi.org/10.4161/hv.5.5.7367

Proteomic study via a non-gel based approach of meningococcal outer membrane vesicle vaccine obtained from strain CU385: a road map for discovering new antigens. / Gil, Jeovanis; Betancourt, L Zaro H; Sardiñas, Gretel et al.
In: Human Vaccines, Vol. 5, Núm. 5, 05.2009, pàg. 347-56.

Producció científica: Contribució a revista › Article › Recerca › Avaluat per experts

TY - JOUR

T1 - Proteomic study via a non-gel based approach of meningococcal outer membrane vesicle vaccine obtained from strain CU385

T2 - a road map for discovering new antigens

AU - Gil, Jeovanis

AU - Betancourt, L Zaro H

AU - Sardiñas, Gretel

AU - Yero, Daniel

AU - Niebla, Olivia

AU - Delgado, Maité

AU - García, Darien

AU - Pajón, Rolando

AU - Sánchez, Aniel

AU - González, Luis J

AU - Padrón, Gabriel

AU - Campa, Concepción

AU - Sotolongo, Franklin

AU - Barberó, Ramón

AU - Guillén, Gerardo

AU - Herrera, Luis

AU - Besada, Vladimir

PY - 2009/5

Y1 - 2009/5

N2 - This work presents the results from a study of the protein composition of outer membrane vesicles from VA-MENGOC-BC (Finlay Institute, Cuba), an available vaccine against serogroup B Neisseria meningitidis. Proteins were identified by means of SCAPE, a 2DE-free method for proteome studies. More than one hundred proteins were detected by tandem liquid chromatographymass spectrometry analysis of fractions enriched in peptides devoid of histidine or arginine residues, providing a detailed description of the vaccine. A bioinformatic analysis of the identified components resulted in the identification of 31 outer membrane proteins and three conserved hypothetical proteins, allowing the cloning, expression, purification and immunological study of two of them (NMB0088 and NMB1796) as new antigens.

AB - This work presents the results from a study of the protein composition of outer membrane vesicles from VA-MENGOC-BC (Finlay Institute, Cuba), an available vaccine against serogroup B Neisseria meningitidis. Proteins were identified by means of SCAPE, a 2DE-free method for proteome studies. More than one hundred proteins were detected by tandem liquid chromatographymass spectrometry analysis of fractions enriched in peptides devoid of histidine or arginine residues, providing a detailed description of the vaccine. A bioinformatic analysis of the identified components resulted in the identification of 31 outer membrane proteins and three conserved hypothetical proteins, allowing the cloning, expression, purification and immunological study of two of them (NMB0088 and NMB1796) as new antigens.

KW - Antigens, Bacterial/analysis

KW - Bacterial Proteins/analysis

KW - Chromatography, Liquid/methods

KW - Cuba

KW - Humans

KW - Meningococcal Vaccines/chemistry

KW - Neisseria meningitidis, Serogroup B/chemistry

KW - Proteome/analysis

KW - Secretory Vesicles/chemistry

KW - Tandem Mass Spectrometry/methods

U2 - 10.4161/hv.5.5.7367

DO - 10.4161/hv.5.5.7367

M3 - Artículo

C2 - 19377283

SN - 1554-8600

VL - 5

SP - 347

EP - 356

JO - Human Vaccines

JF - Human Vaccines

IS - 5

ER -

Proteomic study via a non-gel based approach of meningococcal outer membrane vesicle vaccine obtained from strain CU385: a road map for discovering new antigens

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