Protein kinase CK2 associates to lipid rafts and its pharmacological inhibition enhances neurotransmitter release

Carles Gil; Anton Falqués; Eduard Sarró; Roger Cubí; Juan Blasi; José Aguilera; Emilio Itarte

doi:10.1016/j.febslet.2010.12.029

Protein kinase CK2 associates to lipid rafts and its pharmacological inhibition enhances neurotransmitter release

Carles Gil, Anton Falqués, Eduard Sarró, Roger Cubí, Juan Blasi, José Aguilera, Emilio Itarte

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In the present work we report the presence of protein kinase CK2 in lipid raft preparations from rat brain synaptosomes, obtained after detergent extraction and subsequent isolation of detergent-resistant membranes using sucrose gradient ultracentrifugation. Moreover, the phosphorylation of syntaxin-1 at Ser14, a specific CK2 target, has been detected in lipid rafts, as assessed by a phospho-specific antibody. Treatment with DMAT, a specific CK2 inhibitor, results in a decrease of syntaxin-1 Ser14 phosphorylation in lipid rafts, while the glutamate release from synaptosomes is enhanced. In conclusion, CK2 might control neurotransmitter release by acting on SNARE proteins attached to cholesterol-enriched microdomains. © 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

Idioma original	Anglès
Pàgines (de-a)	414-420
Revista	FEBS Letters
Volum	585
Número	2
DOIs	https://doi.org/10.1016/j.febslet.2010.12.029
Estat de la publicació	Publicada - 21 de gen. 2011

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10.1016/j.febslet.2010.12.029

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title = "Protein kinase CK2 associates to lipid rafts and its pharmacological inhibition enhances neurotransmitter release",

abstract = "In the present work we report the presence of protein kinase CK2 in lipid raft preparations from rat brain synaptosomes, obtained after detergent extraction and subsequent isolation of detergent-resistant membranes using sucrose gradient ultracentrifugation. Moreover, the phosphorylation of syntaxin-1 at Ser14, a specific CK2 target, has been detected in lipid rafts, as assessed by a phospho-specific antibody. Treatment with DMAT, a specific CK2 inhibitor, results in a decrease of syntaxin-1 Ser14 phosphorylation in lipid rafts, while the glutamate release from synaptosomes is enhanced. In conclusion, CK2 might control neurotransmitter release by acting on SNARE proteins attached to cholesterol-enriched microdomains. {\textcopyright} 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.",

keywords = "Cholesterol, Glutamate release, Lipid raft, Protein kinase CK2, Synaptosome, Syntaxin",

author = "Carles Gil and Anton Falqu{\'e}s and Eduard Sarr{\'o} and Roger Cub{\'i} and Juan Blasi and Jos{\'e} Aguilera and Emilio Itarte",

year = "2011",

month = jan,

day = "21",

doi = "10.1016/j.febslet.2010.12.029",

language = "English",

volume = "585",

pages = "414--420",

number = "2",

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TY - JOUR

T1 - Protein kinase CK2 associates to lipid rafts and its pharmacological inhibition enhances neurotransmitter release

AU - Gil, Carles

AU - Falqués, Anton

AU - Sarró, Eduard

AU - Cubí, Roger

AU - Blasi, Juan

AU - Aguilera, José

AU - Itarte, Emilio

PY - 2011/1/21

Y1 - 2011/1/21

N2 - In the present work we report the presence of protein kinase CK2 in lipid raft preparations from rat brain synaptosomes, obtained after detergent extraction and subsequent isolation of detergent-resistant membranes using sucrose gradient ultracentrifugation. Moreover, the phosphorylation of syntaxin-1 at Ser14, a specific CK2 target, has been detected in lipid rafts, as assessed by a phospho-specific antibody. Treatment with DMAT, a specific CK2 inhibitor, results in a decrease of syntaxin-1 Ser14 phosphorylation in lipid rafts, while the glutamate release from synaptosomes is enhanced. In conclusion, CK2 might control neurotransmitter release by acting on SNARE proteins attached to cholesterol-enriched microdomains. © 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

AB - In the present work we report the presence of protein kinase CK2 in lipid raft preparations from rat brain synaptosomes, obtained after detergent extraction and subsequent isolation of detergent-resistant membranes using sucrose gradient ultracentrifugation. Moreover, the phosphorylation of syntaxin-1 at Ser14, a specific CK2 target, has been detected in lipid rafts, as assessed by a phospho-specific antibody. Treatment with DMAT, a specific CK2 inhibitor, results in a decrease of syntaxin-1 Ser14 phosphorylation in lipid rafts, while the glutamate release from synaptosomes is enhanced. In conclusion, CK2 might control neurotransmitter release by acting on SNARE proteins attached to cholesterol-enriched microdomains. © 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

KW - Cholesterol

KW - Glutamate release

KW - Lipid raft

KW - Protein kinase CK2

KW - Synaptosome

KW - Syntaxin

U2 - 10.1016/j.febslet.2010.12.029

DO - 10.1016/j.febslet.2010.12.029

M3 - Article

SN - 0014-5793

VL - 585

SP - 414

EP - 420

JO - FEBS Letters

JF - FEBS Letters

IS - 2

ER -

Protein kinase CK2 associates to lipid rafts and its pharmacological inhibition enhances neurotransmitter release

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