Protein aggregation and soluble aggregate formation screened by a fast microdialysis assay

Verónica Toledo-Rubio, Esther Vazquez, Gemma Platas, Joan Domingo-Espín, Ugutz Unzueta, Elke Steinkamp, Elena García-Fruitós, Neus Ferrer-Miralles, Antonio Villaverde*

*Autor corresponent d’aquest treball

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11 Cites (Scopus)

Resum

Protein aggregation is a major obstacle in recombinant protein production as it reduces the yield of soluble polypeptides. Also, the formation of aggregates occurring in the soluble fraction is more common than formerly expected, and the prevalence of these entities might significantly affect the average quality of the soluble protein species. Usually, the formation of soluble aggregates remains unperceived because analytical methods such as dynamic light scattering are not routinely applied as quality control procedures. The authors have developed a methodologically simple and fast procedure, based on microdialysis and image processing, that reveals the aggregation tendency of a given protein in a specific environment. Because they also show a good correlation between macroscopic aggregation and soluble aggregate formation, the microdialysis approach also permits an estimation of the occurrence of soluble aggregates. © 2010 Society for Biomolecular Sciences.
Idioma originalAnglès
Pàgines (de-a)453-457
Nombre de pàgines5
RevistaJournal of Biomolecular Screening
Volum15
Número4
DOIs
Estat de la publicacióPublicada - 1 d’abr. 2010

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