TY - JOUR
T1 - Protein aggregation and soluble aggregate formation screened by a fast microdialysis assay
AU - Toledo-Rubio, Verónica
AU - Vazquez, Esther
AU - Platas, Gemma
AU - Domingo-Espín, Joan
AU - Unzueta, Ugutz
AU - Steinkamp, Elke
AU - García-Fruitós, Elena
AU - Ferrer-Miralles, Neus
AU - Villaverde, Antonio
PY - 2010/4/1
Y1 - 2010/4/1
N2 - Protein aggregation is a major obstacle in recombinant protein production as it reduces the yield of soluble polypeptides. Also, the formation of aggregates occurring in the soluble fraction is more common than formerly expected, and the prevalence of these entities might significantly affect the average quality of the soluble protein species. Usually, the formation of soluble aggregates remains unperceived because analytical methods such as dynamic light scattering are not routinely applied as quality control procedures. The authors have developed a methodologically simple and fast procedure, based on microdialysis and image processing, that reveals the aggregation tendency of a given protein in a specific environment. Because they also show a good correlation between macroscopic aggregation and soluble aggregate formation, the microdialysis approach also permits an estimation of the occurrence of soluble aggregates. © 2010 Society for Biomolecular Sciences.
AB - Protein aggregation is a major obstacle in recombinant protein production as it reduces the yield of soluble polypeptides. Also, the formation of aggregates occurring in the soluble fraction is more common than formerly expected, and the prevalence of these entities might significantly affect the average quality of the soluble protein species. Usually, the formation of soluble aggregates remains unperceived because analytical methods such as dynamic light scattering are not routinely applied as quality control procedures. The authors have developed a methodologically simple and fast procedure, based on microdialysis and image processing, that reveals the aggregation tendency of a given protein in a specific environment. Because they also show a good correlation between macroscopic aggregation and soluble aggregate formation, the microdialysis approach also permits an estimation of the occurrence of soluble aggregates. © 2010 Society for Biomolecular Sciences.
KW - Dynamic light scattering
KW - Nanoparticles
KW - Protein aggregation
KW - Proto-aggregates
KW - Soluble aggregates
UR - http://www.scopus.com/inward/record.url?scp=77952373543&partnerID=8YFLogxK
U2 - 10.1177/1087057110363911
DO - 10.1177/1087057110363911
M3 - Article
C2 - 20233904
SN - 1087-0571
VL - 15
SP - 453
EP - 457
JO - Journal of Biomolecular Screening
JF - Journal of Biomolecular Screening
IS - 4
ER -