Protection against reinfection with D614- or G614-SARS-CoV-2 isolates in golden Syrian hamster

M. Brustolin; J. Rodon; M.L. Rodríguez de la Concepción; C. Ávila-Nieto; G. Cantero; M. Pérez; N. Te; M. Noguera-Julián; V. Guallar; A. Valencia; N. Roca; N. Izquierdo-Useros; J. Blanco; B. Clotet; A. Bensaid; J. Carrillo; J. Vergara-Alert; J. Segalés

doi:10.1080/22221751.2021.1913974

Protection against reinfection with D614- or G614-SARS-CoV-2 isolates in golden Syrian hamster

M. Brustolin, J. Rodon, M.L. Rodríguez de la Concepción, C. Ávila-Nieto, G. Cantero, M. Pérez, N. Te, M. Noguera-Julián, V. Guallar, A. Valencia, N. Roca, N. Izquierdo-Useros, J. Blanco, B. Clotet, A. Bensaid, J. Carrillo, J. Vergara-Alert, J. Segalés

Departament de Sanitat i d'Anatomia Animals

Producció científica: Contribució a revista › Article › Recerca › Avaluat per experts

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Resum

Les reinfeccions amb SARS-CoV-2 ja s'han documentat en humans, tot i que la seva incidència real és actualment desconeguda. A més de tenir un gran impacte sobre la salut pública, aquest fenomen planteja el dubte de si la immunitat generada per una única infecció és suficient per a proporcionar una immunitat esterilitzadora/protectora contra una reexposició posterior al SARS-CoV-2. El hàmster sirià daurat (Golden Syrian hamster) és un model animal manejable que permet explorar els mecanismes immunològics capaços de contrarestar la COVID-19, ja que recapitula els aspectes patològics dels pacients afectats lleus a moderadament. Aquí, informem que els hàmsters inoculats amb SARS-CoV-2 resolen la infecció en les vies respiratòries superiors i inferiors set dies després de la inoculació amb l'aïllat SARS-CoV-2 Cat01 (G614). Tres setmanes després del repte primari, i malgrat els títols elevats d'anticossos neutralitzants, la meitat dels animals eren susceptibles a una reinfecció tant per aïllats idèntics (Cat01, G614) com variants (WA/1, D614) de SARS-CoV-2. Tanmateix, després de la reinoculació, només els teixits nasals s'infectaven transitòriament amb una replicació viral molt més baixa que l'observada després del primer repte. Aquestes dades indiquen que una infecció primària per SARS-CoV-2 no és suficient per a generar una immunitat esterilitzadora/protectora en models de hàmsters, però protegeix contra la malaltia pulmonar.

Idioma original	Anglès
Revista	Emerging Microbes and Infections
DOIs	https://doi.org/10.1080/22221751.2021.1913974
Estat de la publicació	Publicada - 29 d’abr. 2021

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10.1080/22221751.2021.1913974

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Brustolin, M., Rodon, J., Rodríguez de la Concepción, M. L., Ávila-Nieto, C., Cantero, G., Pérez, M., Te, N., Noguera-Julián, M., Guallar, V., Valencia, A., Roca, N., Izquierdo-Useros, N., Blanco, J., Clotet, B., Bensaid, A., Carrillo, J., Vergara-Alert, J., & Segalés, J. (2021). Protection against reinfection with D614- or G614-SARS-CoV-2 isolates in golden Syrian hamster. Emerging Microbes and Infections. https://doi.org/10.1080/22221751.2021.1913974

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title = "Protection against reinfection with D614- or G614-SARS-CoV-2 isolates in golden Syrian hamster",

abstract = "Reinfections with SARS-CoV-2 have already been documented in humans, although its real incidence is currently unknown. Besides having a great impact on public health, this phenomenon raises the question of immunity generated by a single infection is sufficient to provide sterilizing/protective immunity to a subsequent SARS-CoV-2 re-exposure. The Golden Syrian hamster is a manageable animal model to explore immunological mechanisms able to counteract COVID-19, as it recapitulates pathological aspects of mild to moderately affected patients. Here, we report that SARS-CoV-2-inoculated hamsters resolve infection in the upper and lower respiratory tracts within seven days upon inoculation with the Cat01 (G614) SARS-CoV-2 isolate. Three weeks after the primary challenge, and despite high titres of neutralizing antibodies, half of the animals were susceptible to reinfection by both identical (Cat01, G614) and variant (WA/1, D614) SARS-CoV-2 isolates. However, upon re-inoculation, only nasal tissues were transiently infected with much lower viral replication than those observed after the first inoculation. These data indicate that a primary SARS-CoV-2 infection is not sufficient to elicit a sterilizing immunity in hamster models but protects against lung disease.",

author = "M. Brustolin and J. Rodon and \{Rodr{\'i}guez de la Concepci{\'o}n\}, M.L. and C. {\'A}vila-Nieto and G. Cantero and M. P{\'e}rez and N. Te and M. Noguera-Juli{\'a}n and V. Guallar and A. Valencia and N. Roca and N. Izquierdo-Useros and J. Blanco and B. Clotet and A. Bensaid and J. Carrillo and J. Vergara-Alert and J. Segal{\'e}s",

year = "2021",

month = apr,

day = "29",

doi = "10.1080/22221751.2021.1913974",

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Brustolin, M, Rodon, J, Rodríguez de la Concepción, ML, Ávila-Nieto, C, Cantero, G, Pérez, M, Te, N, Noguera-Julián, M, Guallar, V, Valencia, A, Roca, N, Izquierdo-Useros, N, Blanco, J, Clotet, B, Bensaid, A, Carrillo, J, Vergara-Alert, J & Segalés, J 2021, 'Protection against reinfection with D614- or G614-SARS-CoV-2 isolates in golden Syrian hamster', Emerging Microbes and Infections. https://doi.org/10.1080/22221751.2021.1913974

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T1 - Protection against reinfection with D614- or G614-SARS-CoV-2 isolates in golden Syrian hamster

AU - Brustolin, M.

AU - Rodon, J.

AU - Rodríguez de la Concepción, M.L.

AU - Ávila-Nieto, C.

AU - Cantero, G.

AU - Pérez, M.

AU - Te, N.

AU - Noguera-Julián, M.

AU - Guallar, V.

AU - Valencia, A.

AU - Roca, N.

AU - Izquierdo-Useros, N.

AU - Blanco, J.

AU - Clotet, B.

AU - Bensaid, A.

AU - Carrillo, J.

AU - Vergara-Alert, J.

AU - Segalés, J.

PY - 2021/4/29

Y1 - 2021/4/29

N2 - Reinfections with SARS-CoV-2 have already been documented in humans, although its real incidence is currently unknown. Besides having a great impact on public health, this phenomenon raises the question of immunity generated by a single infection is sufficient to provide sterilizing/protective immunity to a subsequent SARS-CoV-2 re-exposure. The Golden Syrian hamster is a manageable animal model to explore immunological mechanisms able to counteract COVID-19, as it recapitulates pathological aspects of mild to moderately affected patients. Here, we report that SARS-CoV-2-inoculated hamsters resolve infection in the upper and lower respiratory tracts within seven days upon inoculation with the Cat01 (G614) SARS-CoV-2 isolate. Three weeks after the primary challenge, and despite high titres of neutralizing antibodies, half of the animals were susceptible to reinfection by both identical (Cat01, G614) and variant (WA/1, D614) SARS-CoV-2 isolates. However, upon re-inoculation, only nasal tissues were transiently infected with much lower viral replication than those observed after the first inoculation. These data indicate that a primary SARS-CoV-2 infection is not sufficient to elicit a sterilizing immunity in hamster models but protects against lung disease.

AB - Reinfections with SARS-CoV-2 have already been documented in humans, although its real incidence is currently unknown. Besides having a great impact on public health, this phenomenon raises the question of immunity generated by a single infection is sufficient to provide sterilizing/protective immunity to a subsequent SARS-CoV-2 re-exposure. The Golden Syrian hamster is a manageable animal model to explore immunological mechanisms able to counteract COVID-19, as it recapitulates pathological aspects of mild to moderately affected patients. Here, we report that SARS-CoV-2-inoculated hamsters resolve infection in the upper and lower respiratory tracts within seven days upon inoculation with the Cat01 (G614) SARS-CoV-2 isolate. Three weeks after the primary challenge, and despite high titres of neutralizing antibodies, half of the animals were susceptible to reinfection by both identical (Cat01, G614) and variant (WA/1, D614) SARS-CoV-2 isolates. However, upon re-inoculation, only nasal tissues were transiently infected with much lower viral replication than those observed after the first inoculation. These data indicate that a primary SARS-CoV-2 infection is not sufficient to elicit a sterilizing immunity in hamster models but protects against lung disease.

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DO - 10.1080/22221751.2021.1913974

M3 - Article

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SN - 2222-1751

JO - Emerging Microbes and Infections

JF - Emerging Microbes and Infections

ER -

Protection against reinfection with D614- or G614-SARS-CoV-2 isolates in golden Syrian hamster

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