TY - JOUR
T1 - Protection against reinfection with D614- or G614-SARS-CoV-2 isolates in golden Syrian hamster
AU - Brustolin, M.
AU - Rodon, J.
AU - Rodríguez de la Concepción, M.L.
AU - Ávila-Nieto, C.
AU - Cantero, G.
AU - Pérez, M.
AU - Te, N.
AU - Noguera-Julián, M.
AU - Guallar, V.
AU - Valencia, A.
AU - Roca, N.
AU - Izquierdo-Useros, N.
AU - Blanco, J.
AU - Clotet, B.
AU - Bensaid, A.
AU - Carrillo, J.
AU - Vergara-Alert, J.
AU - Segalés, J.
PY - 2021/4/29
Y1 - 2021/4/29
N2 - Reinfections with SARS-CoV-2 have already been documented in humans, although its real incidence is currently unknown. Besides having a great impact on public health, this phenomenon raises the question of immunity generated by a single infection is sufficient to provide sterilizing/protective immunity to a subsequent SARS-CoV-2 re-exposure. The Golden Syrian hamster is a manageable animal model to explore immunological mechanisms able to counteract COVID-19, as it recapitulates pathological aspects of mild to moderately affected patients. Here, we report that SARS-CoV-2-inoculated hamsters resolve infection in the upper and lower respiratory tracts within seven days upon inoculation with the Cat01 (G614) SARS-CoV-2 isolate. Three weeks after the primary challenge, and despite high titres of neutralizing antibodies, half of the animals were susceptible to reinfection by both identical (Cat01, G614) and variant (WA/1, D614) SARS-CoV-2 isolates. However, upon re-inoculation, only nasal tissues were transiently infected with much lower viral replication than those observed after the first inoculation. These data indicate that a primary SARS-CoV-2 infection is not sufficient to elicit a sterilizing immunity in hamster models but protects against lung disease.
AB - Reinfections with SARS-CoV-2 have already been documented in humans, although its real incidence is currently unknown. Besides having a great impact on public health, this phenomenon raises the question of immunity generated by a single infection is sufficient to provide sterilizing/protective immunity to a subsequent SARS-CoV-2 re-exposure. The Golden Syrian hamster is a manageable animal model to explore immunological mechanisms able to counteract COVID-19, as it recapitulates pathological aspects of mild to moderately affected patients. Here, we report that SARS-CoV-2-inoculated hamsters resolve infection in the upper and lower respiratory tracts within seven days upon inoculation with the Cat01 (G614) SARS-CoV-2 isolate. Three weeks after the primary challenge, and despite high titres of neutralizing antibodies, half of the animals were susceptible to reinfection by both identical (Cat01, G614) and variant (WA/1, D614) SARS-CoV-2 isolates. However, upon re-inoculation, only nasal tissues were transiently infected with much lower viral replication than those observed after the first inoculation. These data indicate that a primary SARS-CoV-2 infection is not sufficient to elicit a sterilizing immunity in hamster models but protects against lung disease.
UR - http://www.scopus.com/inward/record.url?eid=2-s2.0-85105213099&partnerID=MN8TOARS
U2 - 10.1080/22221751.2021.1913974
DO - 10.1080/22221751.2021.1913974
M3 - Article
C2 - 33825619
SN - 2222-1751
JO - Emerging Microbes and Infections
JF - Emerging Microbes and Infections
ER -
