Plasma induced reactive oxygen species-dependent cytotoxicity in glioblastoma 3D tumourspheres

Janith Wanigasekara*, Carlos Barcia, Patrick J. Cullen, Brijesh Tiwari, James F. Curtin

*Autor corresponent d’aquest treball

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Resum

The aim of this study was to determine the effects of a pin-to-plate cold atmospheric plasma (CAP) on U-251 MG three-dimensional (3D) glioblastoma spheroids under different conditions. 3D tumorspheres showed higher resistance to the CAP treatment compared to 2D monolayer cells. A single CAP treatment was able to induce cytotoxicity, while multiple CAP treatments augmented this effect. CAP was also able to induce cytotoxicity throughout the tumoursphere, and we identified that reactive oxygen species (ROS) plays a major role, while H2O2 plays a partial role in CAP-induced cytotoxicity in tumourspheres. We conclude that ROS-dependent cytotoxicity is induced uniformly throughout glioblastoma and epidermoid tumourspheres by direct CAP treatment.

Idioma originalAnglès
Número d’article2100157
Nombre de pàgines15
RevistaPlasma Processes and Polymers
Volum19
Número4
DOIs
Estat de la publicacióPublicada - d’abr. 2022

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