Phytochemical profile and antioxidant potential of sea buckthorn leaves : A comprehensive analysis of nutrients and bioactive compounds

Background: Sea buckthorn (Hippophae rhamnoides L.) is a valuable plant with diverse health benefits attributed to its rich phytochemical composition and medicinal properties. In this study, the chemical composition and antioxidant potential of sea buckthorn leaves were investigated using a hydroethanolic extract. Proximate analysis, mineral content determination, antioxidant assays (DPPH and ABTS), and antihemolytic activity were employed to assess the nutritional and functional properties of the extract. Results: Proximate analysis revealed significant amounts of carbohydrates (61.51 g/100 g), proteins (12.49 g/100 g), and fats (13.4 g/100 g). The mineral composition showed high calcium, sodium, and magnesium levels, with potassium being the lowest among the major minerals. The total phenolic content (TPC) was 131.34 mg GAE/g extract DW, and the total flavonoid content (TFC) was 43.00 mg CE/g extract DW. Antioxidant activities linked to the phenolic profile were notable, with DPPH and ABTS assays showing IC50 values of 2.10 mg/g DW and 0.46 mg/g DW, respectively. The phenolic profile, dominated by quinic and rutin, contributed to the observed strong antioxidant activity. Furthermore, the extract exhibited potent reducing power and anti-hemolytic activity. Conclusion: The findings of this study highlight the potential of sea buckthorn leaves as a valuable source of nutrients and bioactive compounds with potent antioxidant properties. Further research is warranted to explore the specific mechanisms of action and potential applications of these compounds in various health-promoting products.