TY - JOUR
T1 - Phylogenetic analysis of an epidemic outbreak of acute hepatitis C in HIV-infected patients by ultra-deep pyrosequencing
AU - Caro-Pérez, Noelia
AU - Martínez-Rebollar, María
AU - Gregori, Josep
AU - Quer, Josep
AU - González, Patricia
AU - Gambato, Martina
AU - García-González, Neris
AU - González-Candelas, Fernando
AU - Costa, Josep
AU - Esteban, Juan Ignacio
AU - Mallolas, Josep
AU - Forns, Xavier
AU - Laguno, Montse
AU - Pérez-del-Pulgar, Sofía
N1 - Funding Information:
This study was funded by ISCIII-Subdirección General de Evaluación, grants PI13/00155 (S.P.-P.), PI15/00151 (X.F. and N.C.-P.), PI13/00456 (J.I.E. and J.Q.) and PI15/00829 (J.Q. and J.G.), integrated in Plan Nacional I + D + I, and co-funded by Fondo Europeo de Desarrollo Regional (FEDER-“Una manera de Hacer Europa”), and Sara Borrell fellowship CM13/00123 (M.M.-R.), co-funded by Fondo Social Europeo (“El FSE invierte en tu futuro”); Secretaria d'Universitats i Recerca del Departament d'Economia i Coneixement, grant 2014_SGR_605 (X.F.); Ministerio de Economía y Competitividad (MINECO), grant BFU2014-58656R (F.G.-C. and N.G.-G.); Generalitat Valenciana, PROMETEO/2016/122 (F.G.-C. and N.G.-G.), and Centro para el Desarrollo Tecnológico Industrial, MINECO, grant IDI-20110115. M.G. was funded by EASL Entry Level Research Fellowship (Dame Sheila Sherlock EASL Fellowship Programme). X.F., J.I.E., S.P.-P., J.Q. and J.G. also received support by the Spanish Health Ministry (Plan Estratégico Nacional contra la Hepatitis C). CIBERehd is funded by the Instituto de Salud Carlos III.
Publisher Copyright:
© 2017 Elsevier B.V.
PY - 2017/7/1
Y1 - 2017/7/1
N2 - Background The incidence of acute hepatitis C (AHC) among HIV-infected men who have sex with men (MSM) has increased significantly in the last 10 years. Several studies point to a social and sexual network of HIV-positive MSM that extends internationally. Objectives The aim of our study was to investigate the dynamics of HCV transmission in an outbreak of AHC in HIV-infected MSM in Barcelona by ultra-deep pyrosequencing. Study design Between 2008 and 2013, 113 cases of AHC in HIV-infected MSM were diagnosed in the Infectious Diseases Unit, Hospital Clínic, Barcelona. Massive sequencing was performed using the Roche 454 GS Junior platform. To define possible transmission networks, maximum likelihood phylogenetic trees were constructed, and levels of genetic diversity within and among patients were compared. Results Among the 70 cases analyzed, we have identified 16 potential clusters of transmission: 8 for genotype 1a (23 cases involved), 1 for genotype 1b (3 cases) and 7 for genotype 4d (27 cases). Although the initial phylogenetic reconstruction suggested a local transmission cluster of HCV gt4d, our approach based on low genetic differentiation did not corroborate it. Indeed, gt4d strains formed 4 independent groups related to patients from other countries. Conclusions Frequent clustering of HIV-positive MSM shows that HCV infection has spread through a local network in Barcelona. This outbreak is related to a large international HCV transmission network among MSM. Public health efforts are needed to reduce HCV transmission among this high-risk group.
AB - Background The incidence of acute hepatitis C (AHC) among HIV-infected men who have sex with men (MSM) has increased significantly in the last 10 years. Several studies point to a social and sexual network of HIV-positive MSM that extends internationally. Objectives The aim of our study was to investigate the dynamics of HCV transmission in an outbreak of AHC in HIV-infected MSM in Barcelona by ultra-deep pyrosequencing. Study design Between 2008 and 2013, 113 cases of AHC in HIV-infected MSM were diagnosed in the Infectious Diseases Unit, Hospital Clínic, Barcelona. Massive sequencing was performed using the Roche 454 GS Junior platform. To define possible transmission networks, maximum likelihood phylogenetic trees were constructed, and levels of genetic diversity within and among patients were compared. Results Among the 70 cases analyzed, we have identified 16 potential clusters of transmission: 8 for genotype 1a (23 cases involved), 1 for genotype 1b (3 cases) and 7 for genotype 4d (27 cases). Although the initial phylogenetic reconstruction suggested a local transmission cluster of HCV gt4d, our approach based on low genetic differentiation did not corroborate it. Indeed, gt4d strains formed 4 independent groups related to patients from other countries. Conclusions Frequent clustering of HIV-positive MSM shows that HCV infection has spread through a local network in Barcelona. This outbreak is related to a large international HCV transmission network among MSM. Public health efforts are needed to reduce HCV transmission among this high-risk group.
KW - Acute hepatitis C
KW - HCV transmission
KW - HIV-coinfection
KW - Ultra-deep pyrosequencing
KW - Acute hepatitis C
KW - HCV transmission
KW - HIV-coinfection
KW - Ultra-deep pyrosequencing
KW - Acute hepatitis C
KW - HCV transmission
KW - HIV-coinfection
KW - Ultra-deep pyrosequencing
UR - http://www.scopus.com/inward/record.url?scp=85019198566&partnerID=8YFLogxK
U2 - 10.1016/j.jcv.2017.05.008
DO - 10.1016/j.jcv.2017.05.008
M3 - Article
C2 - 28521213
AN - SCOPUS:85019198566
SN - 1386-6532
VL - 92
SP - 42
EP - 47
JO - Journal of Clinical Virology
JF - Journal of Clinical Virology
ER -