Northern analysis of highly folded goat α<inf>s1</inf> Casein mRNA

Marta Jansà Pérez; Christine Leroux; Armand Sànchez Bonastre; Patrice Martin

doi:10.1016/S1050-3862(96)80015-5

Northern analysis of highly folded goat α<inf>s1</inf> Casein mRNA

Marta Jansà Pérez, Christine Leroux, Armand Sànchez Bonastre, Patrice Martin

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Resum

Northern blotting using glyoxal to denature a highly folded mRNA, such as goat αs1-Casein E, can lead to the detection of multiple incompletely denatured forms. Formaldehyde appears to be the most suitable agent for Northern blotting due to its effective denaturing capacity and lower toxicity than methylmercuric hydroxide.

Idioma original	Anglès
Pàgines (de-a)	143-145
Revista	Genetic Analysis - Biomolecular Engineering
Volum	12
Número	3-4
DOIs	https://doi.org/10.1016/S1050-3862(96)80015-5
Estat de la publicació	Publicada - 1 de gen. 1996

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10.1016/S1050-3862(96)80015-5

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https://www.scopus.com/pages/publications/0347934876

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@article{06678b6f0370442aa7444a972c9f8e72,

title = "Northern analysis of highly folded goat αs1 Casein mRNA",

abstract = "Northern blotting using glyoxal to denature a highly folded mRNA, such as goat αs1-Casein E, can lead to the detection of multiple incompletely denatured forms. Formaldehyde appears to be the most suitable agent for Northern blotting due to its effective denaturing capacity and lower toxicity than methylmercuric hydroxide.",

keywords = "Denaturing agents, Ethidium bromide staining, Goat α -casein E s1, Northern blotting",

author = "\{Jans{\`a} P{\'e}rez\}, Marta and Christine Leroux and \{S{\`a}nchez Bonastre\}, Armand and Patrice Martin",

year = "1996",

month = jan,

day = "1",

doi = "10.1016/S1050-3862(96)80015-5",

language = "English",

volume = "12",

pages = "143--145",

number = "3-4",

}

TY - JOUR

T1 - Northern analysis of highly folded goat αs1 Casein mRNA

AU - Jansà Pérez, Marta

AU - Leroux, Christine

AU - Sànchez Bonastre, Armand

AU - Martin, Patrice

PY - 1996/1/1

Y1 - 1996/1/1

N2 - Northern blotting using glyoxal to denature a highly folded mRNA, such as goat αs1-Casein E, can lead to the detection of multiple incompletely denatured forms. Formaldehyde appears to be the most suitable agent for Northern blotting due to its effective denaturing capacity and lower toxicity than methylmercuric hydroxide.

AB - Northern blotting using glyoxal to denature a highly folded mRNA, such as goat αs1-Casein E, can lead to the detection of multiple incompletely denatured forms. Formaldehyde appears to be the most suitable agent for Northern blotting due to its effective denaturing capacity and lower toxicity than methylmercuric hydroxide.

KW - Denaturing agents

KW - Ethidium bromide staining

KW - Goat α -casein E s1

KW - Northern blotting

UR - https://www.scopus.com/pages/publications/0347934876

U2 - 10.1016/S1050-3862(96)80015-5

DO - 10.1016/S1050-3862(96)80015-5

M3 - Article

SN - 1050-3862

VL - 12

SP - 143

EP - 145

JO - Genetic Analysis - Biomolecular Engineering

JF - Genetic Analysis - Biomolecular Engineering

IS - 3-4

ER -

Northern analysis of highly folded goat α<inf>s1</inf> Casein mRNA

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