NK cells eliminate Epstein-Barr virus bound to B cells through a specific antibody-mediated uptake

Elisenda Alari-Pahissa*, Michelle Ataya, Ilias Moraitis, Miriam Campos-Ruiz, Mireia Altadill, Aura Muntasell, Anna Moles, Miguel Lopez-Botet*

*Autor corresponent d’aquest treball

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Resum

Epstein Barr virus (EBV) causes a highly prevalent and lifelong infection contributing to the development of some malignancies. In addition to the key role played by T cells in controlling this pathogen, NK cells mediate cytotoxicity and IFNγ production in response to EBVinfected B cells in lytic cycle, both directly and through antibody (Ab)-dependent activation. We recently described that EBV-specific Ab-dependent NK cell interaction with viral particles (VP) bound to B cells triggered degranulation and TNFα secretion but not B cell lysis nor IFNγ production. In this report we show that NK cell activation under these conditions reduced B cell transformation by EBV. NK cells eliminated VP from the surface of B cells through a specific and active process which required tyrosine kinase activation, actin polymerization and Ca2+, being independent of proteolysis and perforin. VP were displayed at the NK cell surface before being internalized and partially shuttled to early endosomes and lysosomes. VP transfer was encompassed by a trogocytosis process including the EBV receptor CD21, together with CD19 and CD20. Our study reveals a novel facet of the antibody- dependent NK cell mediated response to this viral infection.
Idioma originalEnglish
Número d’articlee1009868
Nombre de pàgines23
RevistaPLoS Pathogens
Volum17
Número8
DOIs
Estat de la publicacióPublicada - 20 d’ag. 2021

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