Mycobacterium smegmatis displays the Mycobacterium tuberculosis virulence-related neutral red character when expressing the Rv0577 gene

Núria Andreu, Carlos Y. Soto, Ignasi Roca, Carlos Martín, Isidre Gibert*

*Autor corresponent d’aquest treball

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Resum

Neutral red staining is a cytochemical reaction that has been found to be related to Mycobacterium tuberculosis virulence and, therefore, the component involved in it is thought to be a virulence factor. To study the molecular basis of this reaction we constructed an M. tuberculosis cosmid library in Mycobacterium smegmatis and selected recombinant neutral red positive clones. Heterologous complementation identified Rv0577 as the gene responsible for this trait and we have also shown that it is expressed as a single polycistronic unit together with Rv0576 which could also be involved in the neutral red staining. © 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
Idioma originalEnglish
Pàgines (de-a)283-289
RevistaFEMS Microbiology Letters
Volum231
DOIs
Estat de la publicacióPublicada - 16 de febr. 2004

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