TY - JOUR
T1 - Multimodal HOCl-responsive MEH-PPV nanoparticles for anti-inflammatory imaging and therapy
AU - Villar-Alvarez, Eva
AU - Ruiz-Molina, Daniel
AU - Roscini, Claudio
AU - Wienskowska, Olga
AU - Lorenzo, Julia
AU - Bellacanzone, Christian
AU - Parron-Onate, Sara
AU - Carrascull-Marín, Aleix
N1 - Publisher Copyright:
© 2024 The Authors
PY - 2024/10/15
Y1 - 2024/10/15
N2 - Inflammatory disorders often correlate with an unusually high intracellular production of hypochlorous acid (HOCl). Therefore, its rapid, sensitive, and specific detection is crucial for an early diagnosis and treatment evaluation. While nanoparticles for detection have already been reported, multimodal nanoparticles that simultaneously detect and eliminate reactive oxygen species (including the excess of HOCl) are scarce despite their interest. Herein, we developed highly selective fluorescent nanoparticles using the copolymer poly(2-methoxy-5-(2-ethylhexyloxy)-1, 4-phenylenevinylene) (MEH-PPV), with (MEH@CS) and without a chitosan coating (MEH). The conjugated polymer is oxidized in the presence of HOCl, exhibiting a rapid (in less than 30 s) and sensitive fluorescence turn-off response with a log-log linear HOCl relationship within dynamic ranges of ≈ 0.784–83 μM and ≈ 0.384–55 μM for MEH and MEH@CS NPs, respectively, allowing to monitor basal HOCl levels within the standard physiological concentration range (5 – 25 μM) and its differentiation from overproduction. Moreover, the nanoparticles can encapsulate and release quercetin (a powerful natural scavenger for HOCl), leading not only to monitoring but also to a reduction in pro-inflammatory cytokines of inflammation-stimulated macrophage cells.
AB - Inflammatory disorders often correlate with an unusually high intracellular production of hypochlorous acid (HOCl). Therefore, its rapid, sensitive, and specific detection is crucial for an early diagnosis and treatment evaluation. While nanoparticles for detection have already been reported, multimodal nanoparticles that simultaneously detect and eliminate reactive oxygen species (including the excess of HOCl) are scarce despite their interest. Herein, we developed highly selective fluorescent nanoparticles using the copolymer poly(2-methoxy-5-(2-ethylhexyloxy)-1, 4-phenylenevinylene) (MEH-PPV), with (MEH@CS) and without a chitosan coating (MEH). The conjugated polymer is oxidized in the presence of HOCl, exhibiting a rapid (in less than 30 s) and sensitive fluorescence turn-off response with a log-log linear HOCl relationship within dynamic ranges of ≈ 0.784–83 μM and ≈ 0.384–55 μM for MEH and MEH@CS NPs, respectively, allowing to monitor basal HOCl levels within the standard physiological concentration range (5 – 25 μM) and its differentiation from overproduction. Moreover, the nanoparticles can encapsulate and release quercetin (a powerful natural scavenger for HOCl), leading not only to monitoring but also to a reduction in pro-inflammatory cytokines of inflammation-stimulated macrophage cells.
KW - Meh-ppv
KW - Hypochlorous acid
KW - Theranostic
KW - Quercetin
KW - Fluorescence probe
KW - Inflammatory disease
UR - http://www.scopus.com/inward/record.url?scp=85196289742&partnerID=8YFLogxK
UR - https://www.mendeley.com/catalogue/fb4f8ff1-42dd-3058-b487-4dffcd5d91f5/
U2 - 10.1016/j.snb.2024.136150
DO - 10.1016/j.snb.2024.136150
M3 - Article
AN - SCOPUS:85196289742
SN - 0925-4005
VL - 417
JO - Sensors and Actuators B: Chemical
JF - Sensors and Actuators B: Chemical
M1 - 136150
ER -