Multilocus sequence typing of Treponema pallidum subsp. pallidum in Barcelona

Candela Fernández-Naval; Maider Arando; Mateu Espasa; Andrés Antón; Miguel Fernández-Huerta; Aroa Silgado; Cristina Pinatar; Francesc Zarzuela; Juan José González-López; Judit Serra-Pladevall; Elena Sulleiro; Tomàs Pumarola; Martí Vall-Mayans; Juliana Esperalba

doi:10.2217/fmb-2021-0037

Multilocus sequence typing of Treponema pallidum subsp. pallidum in Barcelona

Candela Fernández-Naval, Maider Arando, Mateu Espasa, Andrés Antón, Miguel Fernández-Huerta, Aroa Silgado, Cristina Pinatar, Francesc Zarzuela, Juan José González-López, Judit Serra-Pladevall, Elena Sulleiro, Tomàs Pumarola, Martí Vall-Mayans, Juliana Esperalba^*

^*Autor corresponent d’aquest treball

Departament de Genètica i de Microbiologia

Producció científica: Contribució a revista › Article › Recerca › Avaluat per experts

6 Cites (Scopus)

Resum

Aim: To implement the multilocus sequence typing (MLST) methodology in syphilis samples previously characterized by enhanced CDC typing (ECDCT) and macrolide resistance. Materials & methods: MLST was performed on genital ulcer and blood samples by analyzing a region of the tp0136, tp0548 and tp0705 loci using Sanger sequencing. Results: Up to 59/85 (69.4%) of genital ulcer and 4/39 (10.3%) of whole blood samples were fully typed. The most frequent profiles were 1.3.1 (56%) and 1.1.1 (11%). All the 1.3.1 samples typed carried the A2058G mutation, responsible for macrolide resistance. MLST and ECDCT showed similar overall typing yields. Conclusion: Several allelic profiles of T. pallidum subsp. pallidum were identified and classified into two major genetic clades in Barcelona. Our results were similar to that described in Europe.

Idioma original	Anglès
Pàgines (de-a)	967-976
Nombre de pàgines	10
Revista	Future Microbiology
Volum	16
Número	13
DOIs	https://doi.org/10.2217/fmb-2021-0037
Estat de la publicació	Publicada - de set. 2021

Accés al document

10.2217/fmb-2021-0037

Altres arxius i enllaços

Enllaç a la publicació de Scopus

Com citar-ho

Fernández-Naval, C., Arando, M., Espasa, M., Antón, A., Fernández-Huerta, M., Silgado, A., Pinatar, C., Zarzuela, F., González-López, J. J., Serra-Pladevall, J., Sulleiro, E., Pumarola, T., Vall-Mayans, M., & Esperalba, J. (2021). Multilocus sequence typing of Treponema pallidum subsp. pallidum in Barcelona. Future Microbiology, 16(13), 967-976. https://doi.org/10.2217/fmb-2021-0037

@article{4fe662d421a74a069204aab7e2512e4e,

title = "Multilocus sequence typing of Treponema pallidum subsp. pallidum in Barcelona",

abstract = "Aim: To implement the multilocus sequence typing (MLST) methodology in syphilis samples previously characterized by enhanced CDC typing (ECDCT) and macrolide resistance. Materials & methods: MLST was performed on genital ulcer and blood samples by analyzing a region of the tp0136, tp0548 and tp0705 loci using Sanger sequencing. Results: Up to 59/85 (69.4%) of genital ulcer and 4/39 (10.3%) of whole blood samples were fully typed. The most frequent profiles were 1.3.1 (56%) and 1.1.1 (11%). All the 1.3.1 samples typed carried the A2058G mutation, responsible for macrolide resistance. MLST and ECDCT showed similar overall typing yields. Conclusion: Several allelic profiles of T. pallidum subsp. pallidum were identified and classified into two major genetic clades in Barcelona. Our results were similar to that described in Europe.",

keywords = "macrolide resistance, molecular epidemiology, molecular typing, multilocus sequence typing, phylogeny, syphilis, Treponema pallidum",

author = "Candela Fern{\'a}ndez-Naval and Maider Arando and Mateu Espasa and Andr{\'e}s Ant{\'o}n and Miguel Fern{\'a}ndez-Huerta and Aroa Silgado and Cristina Pinatar and Francesc Zarzuela and Gonz{\'a}lez-L{\'o}pez, {Juan Jos{\'e}} and Judit Serra-Pladevall and Elena Sulleiro and Tom{\`a}s Pumarola and Mart{\'i} Vall-Mayans and Juliana Esperalba",

note = "Publisher Copyright: {\textcopyright} 2021 ",

year = "2021",

month = sep,

doi = "10.2217/fmb-2021-0037",

language = "English",

volume = "16",

pages = "967--976",

journal = "Future Microbiology",

issn = "1746-0913",

publisher = "Future Medicine Ltd.",

number = "13",

}

Fernández-Naval, C, Arando, M, Espasa, M, Antón, A, Fernández-Huerta, M, Silgado, A, Pinatar, C, Zarzuela, F, González-López, JJ, Serra-Pladevall, J, Sulleiro, E, Pumarola, T, Vall-Mayans, M & Esperalba, J 2021, 'Multilocus sequence typing of Treponema pallidum subsp. pallidum in Barcelona', Future Microbiology, vol. 16, núm. 13, pàg. 967-976. https://doi.org/10.2217/fmb-2021-0037

TY - JOUR

T1 - Multilocus sequence typing of Treponema pallidum subsp. pallidum in Barcelona

AU - Fernández-Naval, Candela

AU - Arando, Maider

AU - Espasa, Mateu

AU - Antón, Andrés

AU - Fernández-Huerta, Miguel

AU - Silgado, Aroa

AU - Pinatar, Cristina

AU - Zarzuela, Francesc

AU - González-López, Juan José

AU - Serra-Pladevall, Judit

AU - Sulleiro, Elena

AU - Pumarola, Tomàs

AU - Vall-Mayans, Martí

AU - Esperalba, Juliana

PY - 2021/9

Y1 - 2021/9

N2 - Aim: To implement the multilocus sequence typing (MLST) methodology in syphilis samples previously characterized by enhanced CDC typing (ECDCT) and macrolide resistance. Materials & methods: MLST was performed on genital ulcer and blood samples by analyzing a region of the tp0136, tp0548 and tp0705 loci using Sanger sequencing. Results: Up to 59/85 (69.4%) of genital ulcer and 4/39 (10.3%) of whole blood samples were fully typed. The most frequent profiles were 1.3.1 (56%) and 1.1.1 (11%). All the 1.3.1 samples typed carried the A2058G mutation, responsible for macrolide resistance. MLST and ECDCT showed similar overall typing yields. Conclusion: Several allelic profiles of T. pallidum subsp. pallidum were identified and classified into two major genetic clades in Barcelona. Our results were similar to that described in Europe.

AB - Aim: To implement the multilocus sequence typing (MLST) methodology in syphilis samples previously characterized by enhanced CDC typing (ECDCT) and macrolide resistance. Materials & methods: MLST was performed on genital ulcer and blood samples by analyzing a region of the tp0136, tp0548 and tp0705 loci using Sanger sequencing. Results: Up to 59/85 (69.4%) of genital ulcer and 4/39 (10.3%) of whole blood samples were fully typed. The most frequent profiles were 1.3.1 (56%) and 1.1.1 (11%). All the 1.3.1 samples typed carried the A2058G mutation, responsible for macrolide resistance. MLST and ECDCT showed similar overall typing yields. Conclusion: Several allelic profiles of T. pallidum subsp. pallidum were identified and classified into two major genetic clades in Barcelona. Our results were similar to that described in Europe.

KW - macrolide resistance

KW - molecular epidemiology

KW - molecular typing

KW - multilocus sequence typing

KW - phylogeny

KW - syphilis

KW - Treponema pallidum

UR - http://www.scopus.com/inward/record.url?scp=85114007823&partnerID=8YFLogxK

U2 - 10.2217/fmb-2021-0037

DO - 10.2217/fmb-2021-0037

M3 - Article

C2 - 34414771

AN - SCOPUS:85114007823

SN - 1746-0913

VL - 16

SP - 967

EP - 976

JO - Future Microbiology

JF - Future Microbiology

IS - 13

ER -

Multilocus sequence typing of Treponema pallidum subsp. pallidum in Barcelona

Resum

Accés al document

Altres arxius i enllaços

Fingerprint

Com citar-ho