Multicenter evaluation of Neo-Sensitabs, a standardized diffusion method for yeast susceptibility testing

Alfonso Javier Carrillo-Muñoz, Lourdes Abarca, Guillermo Quindós, Pilar Arévalo, Fernando Bornay, Francisco Javier Cabañes, José B. Casals, Dolors Estivill, Zoilo Gonzalez-Lama, Isabel Iglesias, Juan Manuel Hernández-Molina, Ma José Linares, Estrella Martín-Mazuelos, Majesús Payá, Manuel Pereiro, Rosario San Millán, Ma Carmen Rubio

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    Resum

    The agar diffusion method Neo-Sensitabs for sensitivity testing, was evaluated with 33 reference strains by fourteen laboratories. Tablets with 5- fluorocytosine, amphotericin B, nystatin, fluconazole, itraconazole, ketoconazole and tioconazole were used on Shadomy modified medium. These tests classify each strain as susceptible, intermediate or resistant to all tested antifungals by measuring the inhibition zone diameters. Intra and interlaboratory reproducibility was studied. Neo-Sensitabs sensitivity for fungi was easy to perform and reliable method with a reproducibility of 97.1% and superior to other commercialized methods, being specially interesting for antifungal susceptibility in vitro testing of triazole derivatives fluconazole and itraconazole.
    Idioma originalAnglès
    Pàgines (de-a)92-96
    RevistaRevista Iberoamericana de Micologia
    Volum16
    Número2
    Estat de la publicacióPublicada - 1 de juny 1999

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