TY - JOUR
T1 - Multicenter evaluation of ATB fungus: A standardized micromethod for yeast susceptibility testing
AU - Quindós, Guillermo
AU - Salesa, Ricardo
AU - Carrillo-Muñoz, Alfonso J.
AU - Lipperheide, Verónica
AU - Jáudenes, Leopoldo
AU - Millán, Rosario San
AU - Torres-Rodríguez, Josep M.
AU - Ponton, José
PY - 1994/1/1
Y1 - 1994/1/1
N2 - The micromethod for yeast susceptibility testing, ATB Fungus, was evaluated with 30 reference strains in three laboratories. Ready-to-use strips with 5-fluorocytosine, amphotericin B, nystatin, miconazole, econazole and ketoconazole were used. The test allowed the categorization of each strain as susceptible, intermediate or resistant to all the antifungals tested, and 5-fluorocytosine and amphotericin B MIC determination. The results were compared with the MIC for each reference strain obtained by a microdilution method on RPMI 1640 buffered with MOPS. The repeatability and intralaboratory and interlaboratory reproducibility were evaluated. ATB Fungus was a reliable and reproducible method with a repeatability of 96.6%, a reproducibility of 95.4% and showed an excellent correlation (91.7%) with reference MICs. © 1994 S. Karger AG, Basel.
AB - The micromethod for yeast susceptibility testing, ATB Fungus, was evaluated with 30 reference strains in three laboratories. Ready-to-use strips with 5-fluorocytosine, amphotericin B, nystatin, miconazole, econazole and ketoconazole were used. The test allowed the categorization of each strain as susceptible, intermediate or resistant to all the antifungals tested, and 5-fluorocytosine and amphotericin B MIC determination. The results were compared with the MIC for each reference strain obtained by a microdilution method on RPMI 1640 buffered with MOPS. The repeatability and intralaboratory and interlaboratory reproducibility were evaluated. ATB Fungus was a reliable and reproducible method with a repeatability of 96.6%, a reproducibility of 95.4% and showed an excellent correlation (91.7%) with reference MICs. © 1994 S. Karger AG, Basel.
KW - Antifungal susceptibility
KW - ATB Fungus
KW - Collaborative study
UR - https://www.scopus.com/pages/publications/0028283669
U2 - 10.1159/000239200
DO - 10.1159/000239200
M3 - Article
SN - 0009-3157
VL - 40
SP - 245
EP - 251
JO - Chemotherapy
JF - Chemotherapy
IS - 4
ER -