TY - JOUR
T1 - MTO: The second member of a Drosophila dual copper-thionein system
AU - Domenech, J.
AU - Palacios, O.
AU - Villarreal, L.
AU - González-Duarte, P.
AU - Capdevila, M.
AU - Atrian, S.
PY - 2003/1/2
Y1 - 2003/1/2
N2 - Drosophila MTO metal binding features were analyzed for comparison with MTN, the paralogous Drosophila metallothionein, and to classify MTO as either zinc- or copper-thionein. This was achieved by a combination of in vivo, in vitro and in silico methodologies. All the results unambiguously classified MTO as a second Drosophila copper-thionein, putting Drosophila forward as the only metazoan in which any zinc-thionein has still to be reported. Interestingly, experimental data only showed minor differences in the coordinative behavior of both MTs, but provided a characteristic spectroscopic fingerprint, revealing the possible binding of chloride anions in certain metal-MTO aggregates. (C) 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.
AB - Drosophila MTO metal binding features were analyzed for comparison with MTN, the paralogous Drosophila metallothionein, and to classify MTO as either zinc- or copper-thionein. This was achieved by a combination of in vivo, in vitro and in silico methodologies. All the results unambiguously classified MTO as a second Drosophila copper-thionein, putting Drosophila forward as the only metazoan in which any zinc-thionein has still to be reported. Interestingly, experimental data only showed minor differences in the coordinative behavior of both MTs, but provided a characteristic spectroscopic fingerprint, revealing the possible binding of chloride anions in certain metal-MTO aggregates. (C) 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.
KW - Cd(II) binding
KW - Cu(I) binding
KW - Drosophila metallothionein
KW - Molecular evolution
KW - Recombinant synthesis
KW - Zn(II) binding
UR - https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=uab_pure&SrcAuth=WosAPI&KeyUT=WOS:000180260000014&DestLinkType=FullRecord&DestApp=WOS_CPL
U2 - 10.1016/S0014-5793(02)03754-7
DO - 10.1016/S0014-5793(02)03754-7
M3 - Article
C2 - 12505162
SN - 0014-5793
VL - 533
SP - 72
EP - 78
JO - FEBS Letters
JF - FEBS Letters
IS - 1-3
ER -