TY - GEN
T1 - Monolithic free flow electrophoresis microfluidic platform fabricated using low temperature co-fired ceramics technology with continuous fluorescence imaging monitoring of the separation process
AU - Couceiro, P.
AU - Alonso-Chamarro, J.
PY - 2016/10/9
Y1 - 2016/10/9
N2 - A monolithic Free Flow Electrophoresis (FFE) microfluidic platform with integrated Au electrodes, fabricated using Low Temperature Co-Fired Ceramics (LTCC) technology, which allows continuous monitoring of the separation process using fluorescence imaging, is presented. The fabrication procedure described allows the construction of hermetically sealed transparent monolithic microfluidic platforms. The presented microfluidic FFE allowed the separation of a mixture of fluorescent dyes, with baseline resolution for all separation peaks, in only 6s. The ability of continuous monitoring opens the possibility for easy optimization of the separation process of real sample mixtures using label free detection of intrinsic fluorescence.
AB - A monolithic Free Flow Electrophoresis (FFE) microfluidic platform with integrated Au electrodes, fabricated using Low Temperature Co-Fired Ceramics (LTCC) technology, which allows continuous monitoring of the separation process using fluorescence imaging, is presented. The fabrication procedure described allows the construction of hermetically sealed transparent monolithic microfluidic platforms. The presented microfluidic FFE allowed the separation of a mixture of fluorescent dyes, with baseline resolution for all separation peaks, in only 6s. The ability of continuous monitoring opens the possibility for easy optimization of the separation process of real sample mixtures using label free detection of intrinsic fluorescence.
KW - Fluorescence imaging
KW - Free Flow Electrophoresis
KW - Low temperature co-fired ceramics
KW - Microfluidic platforms
UR - http://www.scopus.com/inward/record.url?scp=85014288182&partnerID=8YFLogxK
M3 - Other contribution
AN - SCOPUS:85014288182
T3 - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
ER -