TY - JOUR
T1 - Monitoring of sorbitol in Pichia pastoris cultivation applying sequential injection analysis
AU - Horstkotte, Burkhard
AU - Arnau, Carolina
AU - Valero, Francisco
AU - Elsholz, Olaf
AU - Cerdà, Víctor
PY - 2008/10/15
Y1 - 2008/10/15
N2 - A simple and robust analyser system based on sequential injection analysis for the determination of sorbitol in Pichia pastoris MutS phenotype batch fermentation is described. Applicability for the bioprocess monitoring of sorbitol in batch culture medium in a concentration range of 8-1.5 g l-1 with satisfying sorbitol recovery was demonstrated during 60 h. Optimization of physical and chemical parameters is further described. The method proved to be reproducible (R.S.D. < 3%), robust, and fast (17 injections per hour). The SIA sorbitol analyzer showed to be applicable for the monitoring of sorbitol concentration in a mixed substrate P. pastoris fermentation strategy. No interference of methanol was observed. The method is suited for the implementation of sorbitol concentration closed-loop control strategies and for the study of the effect of sorbitol concentration in heterologous protein productivity. © 2008 Elsevier B.V. All rights reserved.
AB - A simple and robust analyser system based on sequential injection analysis for the determination of sorbitol in Pichia pastoris MutS phenotype batch fermentation is described. Applicability for the bioprocess monitoring of sorbitol in batch culture medium in a concentration range of 8-1.5 g l-1 with satisfying sorbitol recovery was demonstrated during 60 h. Optimization of physical and chemical parameters is further described. The method proved to be reproducible (R.S.D. < 3%), robust, and fast (17 injections per hour). The SIA sorbitol analyzer showed to be applicable for the monitoring of sorbitol concentration in a mixed substrate P. pastoris fermentation strategy. No interference of methanol was observed. The method is suited for the implementation of sorbitol concentration closed-loop control strategies and for the study of the effect of sorbitol concentration in heterologous protein productivity. © 2008 Elsevier B.V. All rights reserved.
KW - Batch cultivation
KW - Mixed substrates
KW - Pichia pastoris
KW - Sequential injection analysis
KW - Sorbitol
UR - https://www.scopus.com/pages/publications/50049104720
U2 - 10.1016/j.bej.2008.05.020
DO - 10.1016/j.bej.2008.05.020
M3 - Article
SN - 1369-703X
VL - 42
SP - 77
EP - 83
JO - Biochemical Engineering Journal
JF - Biochemical Engineering Journal
ER -