Molecular MRNA markers to detect and monitor circulating tumour cells in peripheral blood from urothelial carcinoma patients

Mercedes Marin-Aguilera, Lourdes Mengual, Moisés Burset, J. Rios, C. Gazquez, Elisabet Ars, M. J. Ribal, Humberto Villavicencio, A. Alcaraz

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Introduction & Objectives: Detecting haematogenous spread of bladder urothelial
carcinoma (BUC) still represents a major challenge in clinical practice. To test the
efficiency of six mRNA bladder markers in detecting and monitoring BUC haematogenous dissemination.
Material & Methods: Between 2002 and 2004, 317 peripheral blood (PB) samples were collected from 101 patients with BUC that underwent radical cystectomy and from 29 healthy controls. Sequential PB sampling was performed in patients at time of surgery and at 6, 12, 18 and 24 months afterwards. Median patients’ follow-up was 35 months. The presence of disseminated urothelial cells in PB was evaluated by measuring gene expression from pre-amplified (PA) cDNA of KRT20, FXYD3, C10orf116, UPK2, AGR2 and KRT19 genes, using quantitative RT-PCR (qRT-PCR). Gene expression data were correlated with patients’ follow-up and outcome.
Idioma originalUndefined/Unknown
Pàgines (de-a)196
Nombre de pàgines196
RevistaEuropean Urology, Supplements
DOIs
Estat de la publicacióPublicada - 2009

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