TY - JOUR
T1 - Microbe-Dependent Induction of IL-9 by CLA + T Cells in Psoriasis and Relationship with IL-17A
AU - Ruiz-Romeu, Ester
AU - Ferran, Marta
AU - de Jesús-Gil, Carmen
AU - García, Pablo
AU - Sagristà, Marc
AU - Casanova, J. M.
AU - Fernández, J. M.
AU - Chiriac, Anca
AU - Hóllo, Péter
AU - Celada, Antonio
AU - Pujol, Ramon M.
AU - Santamaria-Babí, Luis F.
PY - 2018/3/1
Y1 - 2018/3/1
N2 - © 2017 The Authors IL-9 is present in psoriatic lesions and is produced by lymphocytes. However, it is not known whether this cytokine is induced by relevant pathogenic triggers of psoriasis, such as Streptococcus pyogenes. Here we addressed the production of IL-9 in response to various pathogens in a psoriatic ex vivo model. Extracts of S. pyogenes and Candida albicans triggered the production of IL-9 and also IL-17A and IFN-γ. This induction was dependent on the interaction between CLA + T cells and epidermal cells. Neutralization of IL-9 reduced S. pyogenes-induced IL-17A production by CLA + T cells but had no effect on IFN-γ production. Also, IL-9 increased the survival of circulating psoriatic CLA + T cells. Co-cultures from patients with guttate or plaque psoriasis with S. pyogenes produced similar amounts of IL-9. High cytokine responses in streptococcal-driven guttate patients paralleled peaks in Psoriasis Area Severity Index and anti-streptolysin O levels. Our results confirm that IL-9 promotes inflammation in psoriasis by up-regulating IL-17A production and support the clinical association of the immune response by streptococcal-sensitized CLA + T cells with this cytokine, especially in guttate psoriasis.
AB - © 2017 The Authors IL-9 is present in psoriatic lesions and is produced by lymphocytes. However, it is not known whether this cytokine is induced by relevant pathogenic triggers of psoriasis, such as Streptococcus pyogenes. Here we addressed the production of IL-9 in response to various pathogens in a psoriatic ex vivo model. Extracts of S. pyogenes and Candida albicans triggered the production of IL-9 and also IL-17A and IFN-γ. This induction was dependent on the interaction between CLA + T cells and epidermal cells. Neutralization of IL-9 reduced S. pyogenes-induced IL-17A production by CLA + T cells but had no effect on IFN-γ production. Also, IL-9 increased the survival of circulating psoriatic CLA + T cells. Co-cultures from patients with guttate or plaque psoriasis with S. pyogenes produced similar amounts of IL-9. High cytokine responses in streptococcal-driven guttate patients paralleled peaks in Psoriasis Area Severity Index and anti-streptolysin O levels. Our results confirm that IL-9 promotes inflammation in psoriasis by up-regulating IL-17A production and support the clinical association of the immune response by streptococcal-sensitized CLA + T cells with this cytokine, especially in guttate psoriasis.
U2 - 10.1016/j.jid.2017.08.048
DO - 10.1016/j.jid.2017.08.048
M3 - Article
SN - 0022-202X
VL - 138
SP - 580
EP - 587
JO - Journal of Investigative Dermatology
JF - Journal of Investigative Dermatology
IS - 3
ER -