TY - JOUR
T1 - Magnetic resonance microscopy and correlative histopathology of the infarcted heart
AU - Perez-Terol, Itziar
AU - Rios-Navarro, César
AU - de Dios, Elena
AU - Morales, José Manuel
AU - Gavara Doñate, Jose
AU - Perez-Sole, Nerea
AU - Diaz, Ana
AU - Minana, Gema
AU - Segura-Sabater, Remedios
AU - Bonanad, Clara
AU - Bayés-Genís, Antoni
AU - Husser, Oliver
AU - Monmeneu, José V.
AU - Lopez-Lereu, María P.
AU - Núñez, Julio
AU - Chorro, Francisco J.
AU - Ruiz-Sauri, Amparo
AU - Bodi, Vicente
AU - Monleon, Daniel
PY - 2019
Y1 - 2019
N2 - Delayed enhancement cardiovascular magnetic resonance (MR) is the gold-standard for non-invasive assessment after myocardial infarction (MI). MR microscopy (MRM) provides a level of detail comparable to the macro objective of light microscopy. We used MRM and correlative histopathology to identify infarct and remote tissue in contrast agent-free multi-sequence MRM in swine MI hearts. One control group (n = 3 swine) and two experimental MI groups were formed: 90 min of ischemia followed by 1 week (acute MI = 6 swine) or 1 month (chronic MI = 5 swine) reperfusion. Representative samples of each heart were analysed by contrast agent-free multi-sequence (T1-weighting, T2-weighting, T2*-weighting, T2-mapping, and T2*-mapping). MRM was performed in a 14-Tesla vertical axis imager (Bruker-AVANCE 600 system). Images from MRM and the corresponding histopathological stained samples revealed differences in signal intensities between infarct and remote areas in both MI groups (p-value < 0.001). The multivariable models allowed us to precisely classify regions of interest (acute MI: specificity 92% and sensitivity 80%; chronic MI: specificity 100% and sensitivity 98%). Probabilistic maps based on MRM images clearly delineated the infarcted regions. As a proof of concept, these results illustrate the potential of MRM with correlative histopathology as a platform for exploring novel contrast agent-free MR biomarkers after MI.
AB - Delayed enhancement cardiovascular magnetic resonance (MR) is the gold-standard for non-invasive assessment after myocardial infarction (MI). MR microscopy (MRM) provides a level of detail comparable to the macro objective of light microscopy. We used MRM and correlative histopathology to identify infarct and remote tissue in contrast agent-free multi-sequence MRM in swine MI hearts. One control group (n = 3 swine) and two experimental MI groups were formed: 90 min of ischemia followed by 1 week (acute MI = 6 swine) or 1 month (chronic MI = 5 swine) reperfusion. Representative samples of each heart were analysed by contrast agent-free multi-sequence (T1-weighting, T2-weighting, T2*-weighting, T2-mapping, and T2*-mapping). MRM was performed in a 14-Tesla vertical axis imager (Bruker-AVANCE 600 system). Images from MRM and the corresponding histopathological stained samples revealed differences in signal intensities between infarct and remote areas in both MI groups (p-value < 0.001). The multivariable models allowed us to precisely classify regions of interest (acute MI: specificity 92% and sensitivity 80%; chronic MI: specificity 100% and sensitivity 98%). Probabilistic maps based on MRM images clearly delineated the infarcted regions. As a proof of concept, these results illustrate the potential of MRM with correlative histopathology as a platform for exploring novel contrast agent-free MR biomarkers after MI.
KW - Cardiology
KW - Diagnostic markers
U2 - 10.1038/s41598-019-56436-5
DO - 10.1038/s41598-019-56436-5
M3 - Article
C2 - 31882712
SN - 2045-2322
VL - 9
JO - Scientific reports
JF - Scientific reports
IS - 1
ER -