TY - JOUR
T1 - Lab-in-a-syringe using gold nanoparticles for rapid immunosensing of protein biomarkers
AU - Nunes Pauli, Gisele Elias
AU - De La Escosura-Muñiz, Alfredo
AU - Parolo, Claudio
AU - Helmuth Bechtold, Ivan
AU - Merkoçi, Arben
PY - 2015/1/21
Y1 - 2015/1/21
N2 - © The Royal Society of Chemistry 2015. We have developed a paper and gold nanoparticle (AuNP)-based lab-in-a-syringe (LIS) for immunosensing of biomarkers. This simple diagnostic device features simultaneous sampling and vertical-flow operation, which means that unlike typical immunosensors, it does not suffer from any delay between sampling and detection. It can handle large-volume, low-concentration samples for analysis in diverse applications (e.g. biomedical, environmental, food, etc.). Furthermore, its operating range for sample concentration can be tuned by simply changing the volume of the syringed sample, which enables on-demand limits of detection (LOD). The LIS contains two nitrocellulose pads: the conjugate pad (which captures the analyte) and the detection pad (which signals the presence of the captured analyte) both embedded into reusable plastic cartridges. We demonstrated its efficiency in detecting human IgG (HIgG) (LOD: 1.0 ng mL-1) and prostate-specific antigen (PSA) (spiked urine samples; LOD: 1.9 ng mL-1). In the field, the LIS can be used for complete on-site analysis or to obtain partially analyzed samples (AuNPs with captured analyte) for subsequent detailed testing in specialized laboratories. This journal is
AB - © The Royal Society of Chemistry 2015. We have developed a paper and gold nanoparticle (AuNP)-based lab-in-a-syringe (LIS) for immunosensing of biomarkers. This simple diagnostic device features simultaneous sampling and vertical-flow operation, which means that unlike typical immunosensors, it does not suffer from any delay between sampling and detection. It can handle large-volume, low-concentration samples for analysis in diverse applications (e.g. biomedical, environmental, food, etc.). Furthermore, its operating range for sample concentration can be tuned by simply changing the volume of the syringed sample, which enables on-demand limits of detection (LOD). The LIS contains two nitrocellulose pads: the conjugate pad (which captures the analyte) and the detection pad (which signals the presence of the captured analyte) both embedded into reusable plastic cartridges. We demonstrated its efficiency in detecting human IgG (HIgG) (LOD: 1.0 ng mL-1) and prostate-specific antigen (PSA) (spiked urine samples; LOD: 1.9 ng mL-1). In the field, the LIS can be used for complete on-site analysis or to obtain partially analyzed samples (AuNPs with captured analyte) for subsequent detailed testing in specialized laboratories. This journal is
U2 - 10.1039/c4lc01123f
DO - 10.1039/c4lc01123f
M3 - Article
SN - 1473-0197
VL - 15
SP - 399
EP - 405
JO - Lab on a Chip
JF - Lab on a Chip
IS - 2
ER -