TY - JOUR
T1 - L-ferritin binding to Scara5: A new iron traffic pathway potentially implicated in retinopathy
AU - Mendes-Jorge, Luísa
AU - Ramos, David
AU - Valenҫa, Andreia
AU - López-Luppo, Mariana
AU - Pires, Virgínia Maria Rico
AU - Catita, Joana
AU - Nacher, Victor
AU - Navarro, Marc
AU - Carretero, Ana
AU - Rodriguez-Baeza, Alfonso
AU - Ruberte, Jesús
N1 - Publisher Copyright:
© 2014 Mendes-Jorge et al.
Copyright:
Copyright 2019 Elsevier B.V., All rights reserved.
PY - 2014/9/26
Y1 - 2014/9/26
N2 - Iron is essential in the retina because the heme-containing enzyme guanylate cyclase modulates phototransduction in rods and cones. Transferrin endocytosis is the classical pathway for obtaining iron from the blood circulation in the retina. However, the iron storage protein ferritin has been also recently proposed as an iron carrier. In this study, the presence of Scara5 and its binding to L-ferritin was investigated in the retina. Our results showed that Scara5, the specific receptor for Lferritin, was expressed in mouse and human retinas in many cell types, including endothelial cells. Furthermore, we showed that intravenously injected ferritin crossed the blood retinal barrier through L-ferritin binding to Scara5 in endothelial cells. Thus, suggesting the existence of a new pathway for iron delivery and trafficking in the retina. In a murine model of photoreceptor degeneration, Scara5 was downregulated, pointing out this receptor as a potential player implicated in retinopathy and also as a possible therapeutic target.
AB - Iron is essential in the retina because the heme-containing enzyme guanylate cyclase modulates phototransduction in rods and cones. Transferrin endocytosis is the classical pathway for obtaining iron from the blood circulation in the retina. However, the iron storage protein ferritin has been also recently proposed as an iron carrier. In this study, the presence of Scara5 and its binding to L-ferritin was investigated in the retina. Our results showed that Scara5, the specific receptor for Lferritin, was expressed in mouse and human retinas in many cell types, including endothelial cells. Furthermore, we showed that intravenously injected ferritin crossed the blood retinal barrier through L-ferritin binding to Scara5 in endothelial cells. Thus, suggesting the existence of a new pathway for iron delivery and trafficking in the retina. In a murine model of photoreceptor degeneration, Scara5 was downregulated, pointing out this receptor as a potential player implicated in retinopathy and also as a possible therapeutic target.
UR - http://www.scopus.com/inward/record.url?scp=84907916556&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0106974
DO - 10.1371/journal.pone.0106974
M3 - Article
C2 - 25259650
SN - 1932-6203
VL - 9
JO - PLoS ONE
JF - PLoS ONE
IS - 9
M1 - e106974
ER -