Isolation of Germ Cell Populations from Mouse Testis by Flow Cytometry

Laia Marín-Gual; Covadonga Vara; Aurora Ruiz-Herrera

doi:10.1007/978-1-0716-4750-9_32

Isolation of Germ Cell Populations from Mouse Testis by Flow Cytometry

Laia Marín-Gual, Covadonga Vara, Aurora Ruiz-Herrera^*

^*Autor corresponent d’aquest treball

Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia

Genome Integrity and Instability Group

Producció científica: Capítol de llibre › Capítol › Recerca › Avaluat per experts

Resum

Mammalian spermatogenesis is an intricate process resulting in the formation of male haploid gametes. In order to attain a better understanding of the complexity of this process, the development of a reproducible cytometry method to isolate germ cells is much needed. Here, we describe a reproducible cytometry protocol to isolate germ cells from adult mouse testis. We paid special attention to the optimization of what are considered the most critical steps, including testis disaggregation, antibody titration, cell staining, and germ cell discrimination by FACS.

Idioma original	Anglès
Títol de la publicació	Methods in Molecular Biology
Subtítol de la publicació	Chromoanagenesis Methods and Protocols
Capítol	32
Pàgines	533-547
Nombre de pàgines	15
ISBN (electrònic)	9781071647509
DOIs	https://doi.org/10.1007/978-1-0716-4750-9_32
Estat de la publicació	Publicada - 31 d’ag. 2025

Sèrie de publicacions

Nom	Methods in molecular biology (Clifton, N.J.)
Editor	Humana Press Inc.
ISSN (imprès)	1064-3745

Accés al document

10.1007/978-1-0716-4750-9_32Llicència: C In Copyright

Altres arxius i enllaços

EXPLORANDO LA DINAMICA DE LA ARQUITECTURA GENOMICA Y LA FUNCION GENICA EN LOS VERTEBRADOS
Ruiz Herrera Moreno, A. M. (Investigador/a principal), Deakin, J. (Col.laborador/a), Farré Belmonte, M. (Col.laborador/a), Garcia Haro, F. (Col.laborador/a), Marín Gual, L. (Col.laborador/a), ROBINSON, T. (Col.laborador/a), Sanseverino, W. (Col.laborador/a), WATERS, P. (Col.laborador/a), Pujol i Infantes, G. (Col.laborador/a), Marin Garcia, C. (Col.laborador/a) & Gonzalez Rodelas, L. (Col.laborador/a)
1/09/21 → 28/02/26
Projecte: Projectes i Ajuts a la Recerca
Diseccionando la conservación y función de la organización estructural del genoma de los mamíferos
Ruiz Herrera Moreno, A. M. (Investigador/a principal), Deakin, J. (Col.laborador/a), Garcia Haro, F. (Col.laborador/a), Larkin, D. (Col.laborador/a), Robinson, T. J. (Col.laborador/a), Sanseverino, W. (Col.laborador/a), Ventura Queija, J. (Investigador/a) & Alvarez Gonzalez, L. (Col.laborador/a)
Ministerio de Economía y Competitividad (MINECO), Fons Europeu de Desenvolupament Regional (FEDER), Ministerio de Ciencia e Innovación (MICINN)
1/01/18 → 31/07/21
Projecte: Projectes i Ajuts a la Recerca

Com citar-ho

@inbook{6aca8072864d4501b17e53ae4359ff06,

title = "Isolation of Germ Cell Populations from Mouse Testis by Flow Cytometry",

abstract = "Mammalian spermatogenesis is an intricate process resulting in the formation of male haploid gametes. In order to attain a better understanding of the complexity of this process, the development of a reproducible cytometry method to isolate germ cells is much needed. Here, we describe a reproducible cytometry protocol to isolate germ cells from adult mouse testis. We paid special attention to the optimization of what are considered the most critical steps, including testis disaggregation, antibody titration, cell staining, and germ cell discrimination by FACS.",

keywords = "Flow cytometry, Germ cells, Meiosis, Spermatogenesis, Testis",

author = "Laia Mar{\'i}n-Gual and Covadonga Vara and Aurora Ruiz-Herrera",

note = "Publisher Copyright: {\textcopyright} The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2025.",

year = "2025",

month = aug,

day = "31",

doi = "10.1007/978-1-0716-4750-9\_32",

language = "English",

isbn = "9781071647493",

series = "Methods in molecular biology (Clifton, N.J.)",

publisher = "Humana Press Inc.",

pages = "533--547",

booktitle = "Methods in Molecular Biology",

}

TY - CHAP

T1 - Isolation of Germ Cell Populations from Mouse Testis by Flow Cytometry

AU - Marín-Gual, Laia

AU - Vara, Covadonga

AU - Ruiz-Herrera, Aurora

N1 - Publisher Copyright: © The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2025.

PY - 2025/8/31

Y1 - 2025/8/31

N2 - Mammalian spermatogenesis is an intricate process resulting in the formation of male haploid gametes. In order to attain a better understanding of the complexity of this process, the development of a reproducible cytometry method to isolate germ cells is much needed. Here, we describe a reproducible cytometry protocol to isolate germ cells from adult mouse testis. We paid special attention to the optimization of what are considered the most critical steps, including testis disaggregation, antibody titration, cell staining, and germ cell discrimination by FACS.

AB - Mammalian spermatogenesis is an intricate process resulting in the formation of male haploid gametes. In order to attain a better understanding of the complexity of this process, the development of a reproducible cytometry method to isolate germ cells is much needed. Here, we describe a reproducible cytometry protocol to isolate germ cells from adult mouse testis. We paid special attention to the optimization of what are considered the most critical steps, including testis disaggregation, antibody titration, cell staining, and germ cell discrimination by FACS.

KW - Flow cytometry

KW - Germ cells

KW - Meiosis

KW - Spermatogenesis

KW - Testis

UR - https://www.scopus.com/pages/publications/105015003028

UR - https://www.mendeley.com/catalogue/d3a1c9b0-ff45-3c79-9e2a-0f7da2847cf8/

UR - https://portalrecerca.uab.cat/en/publications/6aca8072-864d-4501-b17e-53ae4359ff06

U2 - 10.1007/978-1-0716-4750-9_32

DO - 10.1007/978-1-0716-4750-9_32

M3 - Chapter

C2 - 40884666

AN - SCOPUS:105015003028

SN - 9781071647493

T3 - Methods in molecular biology (Clifton, N.J.)

SP - 533

EP - 547

BT - Methods in Molecular Biology

ER -

Isolation of Germ Cell Populations from Mouse Testis by Flow Cytometry

Resum

Sèrie de publicacions

Accés al document

Altres arxius i enllaços

Fingerprint

Projectes

EXPLORANDO LA DINAMICA DE LA ARQUITECTURA GENOMICA Y LA FUNCION GENICA EN LOS VERTEBRADOS

Diseccionando la conservación y función de la organización estructural del genoma de los mamíferos

Com citar-ho