TY - JOUR
T1 - Interlaboratory validation of a multiplex qPCR method for the detection of Listeria monocytogenes in a ready-to-eat seafood product
AU - Azinheiro, Sarah
AU - Rodriguez-Lopez, Pedro
AU - Lozano-Leon, Antonio
AU - Guedes, Hugo
AU - Regal, Patricia
AU - Franco, Carlos M.
AU - Cepeda, Alberto
AU - Teixeira, Pilar
AU - Melo, Luis D. R.
AU - Silva, Daniela
AU - Fernandez, Ana
AU - Faria, Marcia
AU - Roumani, Foteini
AU - Herrera, Juan
AU - Prado, Marta
AU - Lopez-Cabo, Marta
AU - Garrido-Maestu, Alejandro
N1 - Publisher Copyright:
© 2023 The Authors
PY - 2023/8
Y1 - 2023/8
N2 - Listeria monocytogenes is a major foodborne pathogen which mainly infects susceptible individuals through the consumption of contaminated foods. To this end, ready-to-eat (RTE) food products are of particular concern as this microorganism is widely distributed, can survive, and even grow, under adverse conditions, and thus must be carefully controlled. In the present study, an interlaboratory ring trial was organized to evaluate an open formula qPCR-based method for the detection of L. monocytogenes. The molecular method was evaluated on a novel RTE seafood product, developed in the framework of a European project, the SEAFOODAGE (EAPA_758/2018). Six laboratories located in Spain and Portugal participated in the study, and the results obtained indicated that this new method presented high diagnostic sensitivity (100%) reaching a low limit of detection (<10 CFU/25 g) with an overall agreement with the reference method, attending to the Cohen's k, of 0.97 that is interpreted as “almost complete agreement”.
AB - Listeria monocytogenes is a major foodborne pathogen which mainly infects susceptible individuals through the consumption of contaminated foods. To this end, ready-to-eat (RTE) food products are of particular concern as this microorganism is widely distributed, can survive, and even grow, under adverse conditions, and thus must be carefully controlled. In the present study, an interlaboratory ring trial was organized to evaluate an open formula qPCR-based method for the detection of L. monocytogenes. The molecular method was evaluated on a novel RTE seafood product, developed in the framework of a European project, the SEAFOODAGE (EAPA_758/2018). Six laboratories located in Spain and Portugal participated in the study, and the results obtained indicated that this new method presented high diagnostic sensitivity (100%) reaching a low limit of detection (<10 CFU/25 g) with an overall agreement with the reference method, attending to the Cohen's k, of 0.97 that is interpreted as “almost complete agreement”.
KW - Alternative methods
KW - Fish products
KW - Interlaboratory validation
KW - Listeria monocytogenes
KW - Ready -to -eat
KW - qPCR
KW - Ready-to-eat
UR - https://www.mendeley.com/catalogue/b4c9f749-2de6-35cb-a16a-973f19ee76a4/
U2 - 10.1016/j.foodcont.2023.109769
DO - 10.1016/j.foodcont.2023.109769
M3 - Article
SN - 0956-7135
VL - 150
JO - Food Control
JF - Food Control
M1 - 109769
ER -