Improved non-radioactive Northern blot protocol for detecting low abundance mRNAs from mammalian tissues

Montserrat Solanas; Raquel Moral; Eduard Escrich

doi:10.1023/A:1005693928291

Improved non-radioactive Northern blot protocol for detecting low abundance mRNAs from mammalian tissues

Montserrat Solanas, Raquel Moral, Eduard Escrich

Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia

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Resum

A rapid and sensitive non-radioactive Northern blot protocol is described which has been optimised in several critical steps. This is based on a formaldehyde-denaturing agarose gel electrophoresis, an alkaline transfer, hybridisation with digoxigenin-DNA probes and detection with a chemiluminescent substrate. This method allows even low abundance mRNAs to be detected in total RNA samples from mammalian tissues.

Idioma original	Anglès
Pàgines (de-a)	263-266
Revista	Biotechnology Letters
Volum	23
DOIs	https://doi.org/10.1023/A:1005693928291
Estat de la publicació	Publicada - 28 de febr. 2001

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10.1023/A:1005693928291

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@article{952c36d210b9439bb18da78955fc5b80,

title = "Improved non-radioactive Northern blot protocol for detecting low abundance mRNAs from mammalian tissues",

abstract = "A rapid and sensitive non-radioactive Northern blot protocol is described which has been optimised in several critical steps. This is based on a formaldehyde-denaturing agarose gel electrophoresis, an alkaline transfer, hybridisation with digoxigenin-DNA probes and detection with a chemiluminescent substrate. This method allows even low abundance mRNAs to be detected in total RNA samples from mammalian tissues.",

keywords = "Chemiluminescence, Digoxigenin, Mammalian tissues, Non-radioactive, Northern blot",

author = "Montserrat Solanas and Raquel Moral and Eduard Escrich",

year = "2001",

month = feb,

day = "28",

doi = "10.1023/A:1005693928291",

language = "English",

volume = "23",

pages = "263--266",

}

TY - JOUR

T1 - Improved non-radioactive Northern blot protocol for detecting low abundance mRNAs from mammalian tissues

AU - Solanas, Montserrat

AU - Moral, Raquel

AU - Escrich, Eduard

PY - 2001/2/28

Y1 - 2001/2/28

N2 - A rapid and sensitive non-radioactive Northern blot protocol is described which has been optimised in several critical steps. This is based on a formaldehyde-denaturing agarose gel electrophoresis, an alkaline transfer, hybridisation with digoxigenin-DNA probes and detection with a chemiluminescent substrate. This method allows even low abundance mRNAs to be detected in total RNA samples from mammalian tissues.

AB - A rapid and sensitive non-radioactive Northern blot protocol is described which has been optimised in several critical steps. This is based on a formaldehyde-denaturing agarose gel electrophoresis, an alkaline transfer, hybridisation with digoxigenin-DNA probes and detection with a chemiluminescent substrate. This method allows even low abundance mRNAs to be detected in total RNA samples from mammalian tissues.

KW - Chemiluminescence

KW - Digoxigenin

KW - Mammalian tissues

KW - Non-radioactive

KW - Northern blot

U2 - 10.1023/A:1005693928291

DO - 10.1023/A:1005693928291

M3 - Article

SN - 0141-5492

VL - 23

SP - 263

EP - 266

JO - Biotechnology Letters

JF - Biotechnology Letters

ER -

Improved non-radioactive Northern blot protocol for detecting low abundance mRNAs from mammalian tissues

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