TY - JOUR
T1 - Impact of Ag2S NPs on soil bacterial community :
T2 - a terrestrial mesocosm approach
AU - Peixoto, S.
AU - Khodaparast, Z.
AU - Cornelis, G.
AU - Lahive, E.
AU - Green Etxabe, A.
AU - Baccaro, Marta
AU - Papadiamantis, A.G.
AU - Gonçalves, S.F.
AU - Lynch, Iseult
AU - Busquets-Fité, Martí
AU - Puntes, Víctor
AU - Loureiro, Susana
AU - Henriques, I.
PY - 2020
Y1 - 2020
N2 - Soils might be a final sink for AgS nanoparticles (NPs). Still, there are limited data on their effects on soil bacterial communities (SBC). To bridge this gap, we investigated the effects of AgS NPs (10 mg kg soil) on the structure and function of SBC in a terrestrial indoor mesocosm, using a multi-species design. During 28 days of exposure, the SBC function-related parameters were analysed in terms of enzymatic activity, community level physiological profile, culture of functional bacterial groups [phosphorous-solubilizing bacteria (P-SB) and heterotrophic bacteria (HB)], and SBC structure was analysed by 16S rRNA gene-targeted denaturing gradient gel electrophoresis. The SBC exposed to AgS NPs showed a significative decrease of functional parameters, such as β-glucosidase activity and L-arginine consumption, and increase of the acid phosphatase activity. At the structural level, significantly lower richness and diversity were detected, but at later exposure times compared to the AgNO treatment, likely because of a low dissolution rate of AgS NPs. In fact, stronger effects were observed in soils spiked with AgNO, in both functional and structural parameters. Changes in SBC structure seem to negatively correlate with parameters related to phosphorous (acid phosphatase activity) and carbon cycling (abundance of HB, P-SB, and β-glucosidase activity). Our results indicate a significant effect of AgS NPs on SBC, specifically on parameters related to carbon and phosphorous cycling, at doses as low as 10 mg kg soil. These effects were only observed after 28 days, highlighting the importance of long-term exposure experiments for slowly dissolving NPs.
AB - Soils might be a final sink for AgS nanoparticles (NPs). Still, there are limited data on their effects on soil bacterial communities (SBC). To bridge this gap, we investigated the effects of AgS NPs (10 mg kg soil) on the structure and function of SBC in a terrestrial indoor mesocosm, using a multi-species design. During 28 days of exposure, the SBC function-related parameters were analysed in terms of enzymatic activity, community level physiological profile, culture of functional bacterial groups [phosphorous-solubilizing bacteria (P-SB) and heterotrophic bacteria (HB)], and SBC structure was analysed by 16S rRNA gene-targeted denaturing gradient gel electrophoresis. The SBC exposed to AgS NPs showed a significative decrease of functional parameters, such as β-glucosidase activity and L-arginine consumption, and increase of the acid phosphatase activity. At the structural level, significantly lower richness and diversity were detected, but at later exposure times compared to the AgNO treatment, likely because of a low dissolution rate of AgS NPs. In fact, stronger effects were observed in soils spiked with AgNO, in both functional and structural parameters. Changes in SBC structure seem to negatively correlate with parameters related to phosphorous (acid phosphatase activity) and carbon cycling (abundance of HB, P-SB, and β-glucosidase activity). Our results indicate a significant effect of AgS NPs on SBC, specifically on parameters related to carbon and phosphorous cycling, at doses as low as 10 mg kg soil. These effects were only observed after 28 days, highlighting the importance of long-term exposure experiments for slowly dissolving NPs.
KW - Silver sulfide nanoparticle
KW - Oil bacterial community
KW - Denaturing gradient gel electrophoresis
KW - Soil enzymatic activity
KW - Community level physiological profile
KW - Indoor Mesocosm
U2 - 10.1016/j.ecoenv.2020.111405
DO - 10.1016/j.ecoenv.2020.111405
M3 - Article
SN - 0147-6513
VL - 206
JO - Ecotoxicology and Environmental Safety
JF - Ecotoxicology and Environmental Safety
ER -