HLADH-catalyzed synthesis of β-amino acids, assisted by continuous electrochemical regeneration of NAD+ in a filter press microreactor

Rossmery A. Rodríguez-Hinestroza; Carmen López; Josep López-Santín; Cheikhou Kane; M. Dolors Benaiges; Theo Tzedakis

doi:10.1016/j.ces.2016.10.010

HLADH-catalyzed synthesis of β-amino acids, assisted by continuous electrochemical regeneration of NAD⁺ in a filter press microreactor

Rossmery A. Rodríguez-Hinestroza, Carmen López, Josep López-Santín, Cheikhou Kane, M. Dolors Benaiges, Theo Tzedakis

Departament d'Enginyeria Química, Biològica i Ambiental

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© 2016 Elsevier Ltd This work focuses on the use of electrochemical microreactors, applied to the direct electroregeneration of nicotinamide dinucleotide (NAD+) used in-situ for the enzymatically-assisted oxidation of the β-alanine. Mechanistic and chemical/electrochemical reactor approaches were investigated, to achieve satisfactory conversions (>80%) of the substrate in reasonable (1–2 days) reaction times; no fouling observed of the gold anode. An original method was proposed to limit the complexation effect of the produced β-alanine to the enzyme. Simulations of the overall system were achieved and an estimation of the required kinetic and Michaelis constants was proposed.

Idioma original	Anglès
Pàgines (de-a)	196-207
Revista	Chemical Engineering Science
Volum	158
DOIs	https://doi.org/10.1016/j.ces.2016.10.010
Estat de la publicació	Publicada - 2 de febr. 2017

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10.1016/j.ces.2016.10.010

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@article{4c84a694e20343bfb06a10b1f467c9d3,

title = "HLADH-catalyzed synthesis of β-amino acids, assisted by continuous electrochemical regeneration of NAD+ in a filter press microreactor",

abstract = "{\textcopyright} 2016 Elsevier Ltd This work focuses on the use of electrochemical microreactors, applied to the direct electroregeneration of nicotinamide dinucleotide (NAD+) used in-situ for the enzymatically-assisted oxidation of the β-alanine. Mechanistic and chemical/electrochemical reactor approaches were investigated, to achieve satisfactory conversions (>80%) of the substrate in reasonable (1–2 days) reaction times; no fouling observed of the gold anode. An original method was proposed to limit the complexation effect of the produced β-alanine to the enzyme. Simulations of the overall system were achieved and an estimation of the required kinetic and Michaelis constants was proposed.",

keywords = "Electrochemical NAD regeneration +, Electrochemical microreactor, Horse-liver alcohol dehydrogenase, β-alanine synthesis, simulation, β-amino acids",

author = "Rodr{\'i}guez-Hinestroza, {Rossmery A.} and Carmen L{\'o}pez and Josep L{\'o}pez-Sant{\'i}n and Cheikhou Kane and Benaiges, {M. Dolors} and Theo Tzedakis",

year = "2017",

month = feb,

day = "2",

doi = "10.1016/j.ces.2016.10.010",

language = "English",

volume = "158",

pages = "196--207",

journal = "Chemical Engineering Science",

issn = "0009-2509",

publisher = "Elsevier BV",

}

HLADH-catalyzed synthesis of β-amino acids, assisted by continuous electrochemical regeneration of NAD⁺ in a filter press microreactor. / Rodríguez-Hinestroza, Rossmery A.; López, Carmen; López-Santín, Josep et al.
In: Chemical Engineering Science, Vol. 158, 02.02.2017, pàg. 196-207.

Producció científica: Contribució a revista › Article › Recerca › Avaluat per experts

TY - JOUR

T1 - HLADH-catalyzed synthesis of β-amino acids, assisted by continuous electrochemical regeneration of NAD+ in a filter press microreactor

AU - Rodríguez-Hinestroza, Rossmery A.

AU - López, Carmen

AU - López-Santín, Josep

AU - Kane, Cheikhou

AU - Benaiges, M. Dolors

AU - Tzedakis, Theo

PY - 2017/2/2

Y1 - 2017/2/2

N2 - © 2016 Elsevier Ltd This work focuses on the use of electrochemical microreactors, applied to the direct electroregeneration of nicotinamide dinucleotide (NAD+) used in-situ for the enzymatically-assisted oxidation of the β-alanine. Mechanistic and chemical/electrochemical reactor approaches were investigated, to achieve satisfactory conversions (>80%) of the substrate in reasonable (1–2 days) reaction times; no fouling observed of the gold anode. An original method was proposed to limit the complexation effect of the produced β-alanine to the enzyme. Simulations of the overall system were achieved and an estimation of the required kinetic and Michaelis constants was proposed.

AB - © 2016 Elsevier Ltd This work focuses on the use of electrochemical microreactors, applied to the direct electroregeneration of nicotinamide dinucleotide (NAD+) used in-situ for the enzymatically-assisted oxidation of the β-alanine. Mechanistic and chemical/electrochemical reactor approaches were investigated, to achieve satisfactory conversions (>80%) of the substrate in reasonable (1–2 days) reaction times; no fouling observed of the gold anode. An original method was proposed to limit the complexation effect of the produced β-alanine to the enzyme. Simulations of the overall system were achieved and an estimation of the required kinetic and Michaelis constants was proposed.

KW - Electrochemical NAD regeneration +

KW - Electrochemical microreactor

KW - Horse-liver alcohol dehydrogenase

KW - β-alanine synthesis, simulation

KW - β-amino acids

U2 - 10.1016/j.ces.2016.10.010

DO - 10.1016/j.ces.2016.10.010

M3 - Article

SN - 0009-2509

VL - 158

SP - 196

EP - 207

JO - Chemical Engineering Science

JF - Chemical Engineering Science

ER -

HLADH-catalyzed synthesis of β-amino acids, assisted by continuous electrochemical regeneration of NAD+ in a filter press microreactor

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HLADH-catalyzed synthesis of β-amino acids, assisted by continuous electrochemical regeneration of NAD⁺ in a filter press microreactor