TY - JOUR
T1 - High-performance liquid chromatography ganglioside pattern of the AB feline blood group
AU - Silvestre-Ferreira, Ana C.
AU - Masso, Oriol
AU - Pastor, Josep
PY - 2011/12/1
Y1 - 2011/12/1
N2 - Feline blood types A, B, and AB are known for several years. However, the exact mode of inheritance of type AB blood is still unknown. The use of Triticum vulgaris lectin and the anti-A serum is by far the most common method for feline blood typing. T. vulgaris lectin is known to identify a sialoglycoprotein in type B and AB animals, and it also recognizes N-acetylneuraminic acid gangliosides derivates. On the other hand, anti-A serum recognizes N-glycolylneuraminic acid derivates. In the present work, high-performance liquid chromatography (HPLC), thin-layer chromatography (TLC), and TLC immunostaining with monoclonal antibodies P3 and 14F9 were used. N-acetylneuraminic and N-glycolylneuraminic derivate gangliosides were confirmed as major determinants in feline blood types However, by the use of HPLC, six ganglioside patterns were found. Type B cats showed only one pattern, but type A animals revealed the presence of three distinct patterns. Serological type AB cats presented two patterns that overlap with the three from type A cats. In conclusion, ganglioside patterns from A and AB blood types cats diverged from the serological classification. © 2010 Springer-Verlag London Limited.
AB - Feline blood types A, B, and AB are known for several years. However, the exact mode of inheritance of type AB blood is still unknown. The use of Triticum vulgaris lectin and the anti-A serum is by far the most common method for feline blood typing. T. vulgaris lectin is known to identify a sialoglycoprotein in type B and AB animals, and it also recognizes N-acetylneuraminic acid gangliosides derivates. On the other hand, anti-A serum recognizes N-glycolylneuraminic acid derivates. In the present work, high-performance liquid chromatography (HPLC), thin-layer chromatography (TLC), and TLC immunostaining with monoclonal antibodies P3 and 14F9 were used. N-acetylneuraminic and N-glycolylneuraminic derivate gangliosides were confirmed as major determinants in feline blood types However, by the use of HPLC, six ganglioside patterns were found. Type B cats showed only one pattern, but type A animals revealed the presence of three distinct patterns. Serological type AB cats presented two patterns that overlap with the three from type A cats. In conclusion, ganglioside patterns from A and AB blood types cats diverged from the serological classification. © 2010 Springer-Verlag London Limited.
KW - Blood types
KW - Blood typing
KW - Feline
KW - HPLC
KW - TLC
UR - https://www.scopus.com/pages/publications/80355147981
U2 - 10.1007/s00580-010-1039-9
DO - 10.1007/s00580-010-1039-9
M3 - Article
SN - 1618-5641
VL - 20
SP - 597
EP - 605
JO - Comparative Clinical Pathology
JF - Comparative Clinical Pathology
ER -