Heterogeneous nuclear ribonucleoprotein A2 interacts with protein kinase CK2

The catalytic subunit of protein kinase CK2 (CK2α) was found associated with heterogeneous nuclear ribonucleoprotein particles (hnRNPs) that contain the core proteins A2 and C1-C2. High levels of CK2 activity were also detected in these complexes. Phosphopeptide patterns of hnRNP A2 phosphorylated in vivo and in vitro by protein kinase CK2 were similar, suggesting that this kinase can phosphorylate hnRNPA2 in vivo. Binding experiments using human recombinant hnRNP A2, free human recombinant CK2α or CK2β subunits, reconstituted CK2 holoenzyme and purified native rat liver CK2 indicated that hnRNP A2 associated with both catalytic and regulatory CK2 subunits, and that the interaction was independent of the presence of RNA. However, the capability of hnRNP A2 to bind to CK2 holoenzyme was lower than its binding to the isolated subunits. These data indicate that the association of CK2α with CK2β interferes with the subsequent binding of hnRNP A2. HnRNP A2 inhibited the autophosphorylation of CK2β. This effect was stronger with reconstituted human recombinant CK2 than with purified native rat liver CK2.