TY - JOUR
T1 - Hepatic lipid metabolism changes in short- and long-term prehepatic portal hypertensive rats
AU - Aller, Maria Angeles
AU - Angulo, Alejandro
AU - Sńchez-Patán, Fernando
AU - Calderón, Ana
AU - Arias, Jaime
AU - Vara, Elena
AU - García, Cruz
AU - Nava, Maria Paz
AU - Vergara, Patri
PY - 2006/11/14
Y1 - 2006/11/14
N2 - Aim: To verify the impairment of the hepatic lipid metabolism in prehepatic portal hypertension. Methods: The concentrations of free fatty acids, diacylglycerol, triglycerides, and phospholipids were assayed by using D-[U-14C] glucose incorporation in the different lipid fractions and thin-layer chromatography and cholesterol was measured by spectrophotometry, in liver samples of Wistar rats with partial portal vein ligation at short- (1 mo) and long-term (1 year) (i.e. portal hypertensive rats) and the control rats. Results: In the portal hypertensive rats, liver phospholipid synthesis significantly decreased (7.42 ± 0.50 vs 4.70 ± 0.44 nCi/g protein; P < 0.01) and was associated with an increased synthesis of free fatty acids (2.08 ± 0.14 vs 3.36 ± 0.33 nCi/g protein; P < 0.05), diacylglycerol (1.93 ± 0.2 vs 2.26 ± 0.28 nCi /g protein), triglycerides (2.40 ± 0.30 vs 4.49 ± 0.15 nCi /g protein) and cholesterol (24.28 ± 2.12 vs 57.66 ± 3.26 mg/g protein; P < 0.01). Conclusion: Prehepatic portal hypertension in rats impairs the liver lipid metabolism. This impairment consists in an increase in lipid deposits (triglycerides, diacylglycerol and cholesterol) in the liver, accompanied by a decrease in phospholipid synthesi. © 2006 The WJG Press. All rights reserved.
AB - Aim: To verify the impairment of the hepatic lipid metabolism in prehepatic portal hypertension. Methods: The concentrations of free fatty acids, diacylglycerol, triglycerides, and phospholipids were assayed by using D-[U-14C] glucose incorporation in the different lipid fractions and thin-layer chromatography and cholesterol was measured by spectrophotometry, in liver samples of Wistar rats with partial portal vein ligation at short- (1 mo) and long-term (1 year) (i.e. portal hypertensive rats) and the control rats. Results: In the portal hypertensive rats, liver phospholipid synthesis significantly decreased (7.42 ± 0.50 vs 4.70 ± 0.44 nCi/g protein; P < 0.01) and was associated with an increased synthesis of free fatty acids (2.08 ± 0.14 vs 3.36 ± 0.33 nCi/g protein; P < 0.05), diacylglycerol (1.93 ± 0.2 vs 2.26 ± 0.28 nCi /g protein), triglycerides (2.40 ± 0.30 vs 4.49 ± 0.15 nCi /g protein) and cholesterol (24.28 ± 2.12 vs 57.66 ± 3.26 mg/g protein; P < 0.01). Conclusion: Prehepatic portal hypertension in rats impairs the liver lipid metabolism. This impairment consists in an increase in lipid deposits (triglycerides, diacylglycerol and cholesterol) in the liver, accompanied by a decrease in phospholipid synthesi. © 2006 The WJG Press. All rights reserved.
KW - Cholesterol
KW - Diacylglycerides
KW - Free fatty acids
KW - Phospholipids
KW - Portal hypertension
KW - Triglycerides
U2 - 10.3748/wjg.v12.i42.6828
DO - 10.3748/wjg.v12.i42.6828
M3 - Article
SN - 1007-9327
VL - 12
SP - 6828
EP - 6834
JO - World Journal of Gastroenterology
JF - World Journal of Gastroenterology
IS - 42
ER -