TY - JOUR
T1 - Genomic imbalances and patterns of karyotypic variability in mantle-cell lymphoma cell lines
AU - Camps, Jordi
AU - Salaverria, Itziar
AU - Garcia, Maria J.
AU - Prat, Esther
AU - Beà, Sílvia
AU - Pole, Jessica C.
AU - Hernández, Lluis
AU - Rey, Javier Del
AU - Cigudosa, Juan Cruz
AU - Bernués, Marta
AU - Caldas, Carlos
AU - Colomer, Dolors
AU - Miró, Rosa
AU - Campo, Elías
PY - 2006/8/1
Y1 - 2006/8/1
N2 - Mantle-cell lymphoma (MCL) is genetically characterized by 11q13 chromosomal translocations involving the CCND1 gene. We have characterized five MCL cell lines, JVM-2, GRANTA-519, REC-1, JEKO-1, and NCEB-1, combining metaphase and array comparative genomic hybridization, multicolor-FISH, and molecular analysis. Our results revealed common gained regions at 2p14, 9q31.2-qter, 11q13.1-q21, 13q14-q21.2, 13q34-qter and 18q21.1-q22.1, and losses at 1p21.2-p31.1, 2p11.2, 8p21.2-pter, 9p21.3-pter, 11q23.3-qter, 17p11.2-pter, and 17q21.2-q22.2. All cell lines except JVM-2, displayed moderate or high numerical chromosome instability. In addition, an ongoing level of chromosome rearrangements was observed in REC-1. Surprisingly, NCEB-1 carried several stable mouse chromosomes and showed expression of both human and murine bcl-2 protein. Our findings indicate that these cell lines represent three patterns of chromosome evolution in MCL and may be useful to understand the pathogenesis of this neoplasm. © 2005 Elsevier Ltd. All rights reserved.
AB - Mantle-cell lymphoma (MCL) is genetically characterized by 11q13 chromosomal translocations involving the CCND1 gene. We have characterized five MCL cell lines, JVM-2, GRANTA-519, REC-1, JEKO-1, and NCEB-1, combining metaphase and array comparative genomic hybridization, multicolor-FISH, and molecular analysis. Our results revealed common gained regions at 2p14, 9q31.2-qter, 11q13.1-q21, 13q14-q21.2, 13q34-qter and 18q21.1-q22.1, and losses at 1p21.2-p31.1, 2p11.2, 8p21.2-pter, 9p21.3-pter, 11q23.3-qter, 17p11.2-pter, and 17q21.2-q22.2. All cell lines except JVM-2, displayed moderate or high numerical chromosome instability. In addition, an ongoing level of chromosome rearrangements was observed in REC-1. Surprisingly, NCEB-1 carried several stable mouse chromosomes and showed expression of both human and murine bcl-2 protein. Our findings indicate that these cell lines represent three patterns of chromosome evolution in MCL and may be useful to understand the pathogenesis of this neoplasm. © 2005 Elsevier Ltd. All rights reserved.
KW - Cell cross-contamination
KW - Cell fusion
KW - Cell lines
KW - Chromosomal instability
KW - Comparative genomic hybridization
KW - Mantle-cell lymphoma
KW - Multicolor-FISH
U2 - 10.1016/j.leukres.2005.11.013
DO - 10.1016/j.leukres.2005.11.013
M3 - Article
SN - 0145-2126
VL - 30
SP - 923
EP - 934
JO - Leukemia Research
JF - Leukemia Research
IS - 8
ER -