G-banding improvement for the MAC method

Alejandra Pérez, Francesc Solé, Rosa Caballín, Nuria Tarrida, Soledad Woessner

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Resum

Satisfactory identification of the neoplastic cell as well as an optimal G-banding of its chromosomes are routinely obtained in our laboratory using a modification of the method described by Knuutila et al. [1]. Mitotic cells are first identified by either cytochemical or immunological staining. For G-banding, the preparations are then destained with methanol: acetic acid, treated with 2 × SSC at 65°C and restained with Wright stain. © 1991.
Idioma originalAnglès
Pàgines (de-a)41-44
RevistaCancer Genetics and Cytogenetics
Volum51
DOIs
Estat de la publicacióPublicada - 1 de gen. 1991

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