TY - JOUR
T1 - Fia Fluorimetric Determination Of Calcium Pantothenate. Validation And Quantitation In Multivitamin Preparations
AU - Blanco, M.
AU - Coello, J.
AU - Iturriaga, H.
AU - Maspoch, S.
AU - Pages, J.
PY - 1995/1/1
Y1 - 1995/1/1
N2 - An FIA method for the determination of calcium pantothenate, one of the components of vitamin B complex, is proposed. The method is based on the alkaline hydrolysis of the analyte to B-alanine and subsequent derivatization to a l-alkylthio-2-alkylisoindole, the fluorescence of which is monitored at x — 350 nm and Xern = 455 nm. Chemical and physical variables influencing the fluorescence signal were optimized and the method was validated as regards accuracy, repeatability and reproducibility. The proposed method features a broad linear determination range (0.2-40 mg/L) and a throughput of 25 samples/h. It was applied to the determination of calcium pantothenate in multivitamin preparations with satisfactory results. © 1995, Taylor & Francis Group, LLC. All rights reserved.
AB - An FIA method for the determination of calcium pantothenate, one of the components of vitamin B complex, is proposed. The method is based on the alkaline hydrolysis of the analyte to B-alanine and subsequent derivatization to a l-alkylthio-2-alkylisoindole, the fluorescence of which is monitored at x — 350 nm and Xern = 455 nm. Chemical and physical variables influencing the fluorescence signal were optimized and the method was validated as regards accuracy, repeatability and reproducibility. The proposed method features a broad linear determination range (0.2-40 mg/L) and a throughput of 25 samples/h. It was applied to the determination of calcium pantothenate in multivitamin preparations with satisfactory results. © 1995, Taylor & Francis Group, LLC. All rights reserved.
KW - FIA
KW - Fluorimetric detection
KW - Multivitamin preparations
KW - Pantothenate
U2 - 10.1080/00032719508001427
DO - 10.1080/00032719508001427
M3 - Article
SN - 0003-2719
VL - 28
SP - 821
EP - 833
JO - Analytical Letters
JF - Analytical Letters
IS - 5
ER -