TY - JOUR
T1 - FGF-4 increases in vitro expansion rate of human adult bone marrow-derived mesenchymal stem cells
AU - Farré, Jordi
AU - Roura, Santiago
AU - Prat-Vidal, Cristina
AU - Soler-Botija, Carolina
AU - Llach, Anna
AU - Molina, Cristina E.
AU - Hove-Madsen, Leif
AU - Cairó, Jordi J.
AU - Gòdia, Francesc
AU - Bragós, Ramon
AU - Cinca, Juan
AU - Bayes-Genis, Antoni
PY - 2007/4/1
Y1 - 2007/4/1
N2 - Human bone marrow-derived mesenchymal stem cells (MSCs) exhibit limited in vitro growth. Fibroblast growth factors (FGFs) elicit a variety of biological responses, such as cell proliferation, differentiation and migration. FGF-4 represents one of the FGFs with the highest cell mitogenic activity. We studied the effect of FGF-4 on MSCs growth and pluripotency. MSCs duplication time (Td) was significantly reduced with FGF-4 compared to controls (2.2 ± 0.2 vs. 4.1 ± 0.2 days, respectively; p = 0.03) while BMP-2 and SCF-1 did not exert a significant growth effect. MSC expression of surface markers, differentiation into adipogenic and osteogenic lineages, and baseline expression of cardiomyogenic genes were unaffected by FGF-4. In summary, exogenous FGF-4 increases the rate at which MSC proliferate and has no significant effect on MSC pluripotency.
AB - Human bone marrow-derived mesenchymal stem cells (MSCs) exhibit limited in vitro growth. Fibroblast growth factors (FGFs) elicit a variety of biological responses, such as cell proliferation, differentiation and migration. FGF-4 represents one of the FGFs with the highest cell mitogenic activity. We studied the effect of FGF-4 on MSCs growth and pluripotency. MSCs duplication time (Td) was significantly reduced with FGF-4 compared to controls (2.2 ± 0.2 vs. 4.1 ± 0.2 days, respectively; p = 0.03) while BMP-2 and SCF-1 did not exert a significant growth effect. MSC expression of surface markers, differentiation into adipogenic and osteogenic lineages, and baseline expression of cardiomyogenic genes were unaffected by FGF-4. In summary, exogenous FGF-4 increases the rate at which MSC proliferate and has no significant effect on MSC pluripotency.
KW - Cardiomyogenic
KW - FGF-4
KW - Growth
KW - Mesenchymal stem cells
KW - Pluripotency
U2 - 10.1080/08977190701345200
DO - 10.1080/08977190701345200
M3 - Article
SN - 0897-7194
VL - 25
SP - 71
EP - 76
JO - Growth Factors
JF - Growth Factors
ER -