TY - JOUR
T1 - Ferromagnetic levan composite
T2 - An affinity matrix to purify lectin
AU - Angeli, Renata
AU - MacIel, Jackeline C.
AU - Araújo, Flávia F.B.
AU - Paiva, Patrícia M.G.
AU - Calazans, Glícia M.T.
AU - Valente, Ana Paula
AU - Almeida, Fábio C.L.
AU - Coelho, Luana C.B.B.
AU - Carvalho, Luiz B.
AU - Silva, Maria Da Paz C.
AU - Dos Santos Correia, Maria Tereza
AU - Varejao Nogueira, Nathalia
PY - 2009
Y1 - 2009
N2 - A simple and inexpensive procedure usedmagnetite and levan to synthesize a composite recovered by amagnetic field. Lectins from Canavalia ensiformis (Con A) and Cratylia mollis (Cramoll 1 and Cramoll 1,4) did bind specifically to composite. The magnetic property of derivative favored washing out contaminating proteins and recovery of pure lectins with glucose elution. Cramoll 1 was purified by this affinity binding procedure in two steps instead of a previous three-step protocol with ammonium sulfate fractionation, affinity chromatography on Sephadex G-75, and ion exchange chromatography through a CM-cellulose column.
AB - A simple and inexpensive procedure usedmagnetite and levan to synthesize a composite recovered by amagnetic field. Lectins from Canavalia ensiformis (Con A) and Cratylia mollis (Cramoll 1 and Cramoll 1,4) did bind specifically to composite. The magnetic property of derivative favored washing out contaminating proteins and recovery of pure lectins with glucose elution. Cramoll 1 was purified by this affinity binding procedure in two steps instead of a previous three-step protocol with ammonium sulfate fractionation, affinity chromatography on Sephadex G-75, and ion exchange chromatography through a CM-cellulose column.
UR - http://www.scopus.com/inward/record.url?scp=68949143047&partnerID=8YFLogxK
U2 - 10.1155/2009/179106
DO - 10.1155/2009/179106
M3 - Article
C2 - 19547713
AN - SCOPUS:68949143047
SN - 1110-7243
VL - 2009
JO - Journal of Biomedicine and Biotechnology
JF - Journal of Biomedicine and Biotechnology
M1 - 179106
ER -