TY - JOUR
T1 - Eye bank and theatre factors for positive microbiological culture of corneoscleral rim and cornea storage medium in the real-world
AU - Sabater-Cruz, Noelia
AU - Otero, Nausica
AU - Dotti-Boada, Marina
AU - Ríos, José
AU - Gris, Oscar
AU - Güell, José L.
AU - Vilarrodona, Ana
AU - Casaroli-Marano, Ricardo P.
N1 - © 2021. The Author(s), under exclusive licence to The Royal College of Ophthalmologists.
PY - 2021/1/19
Y1 - 2021/1/19
N2 - OBJECTIVES: To evaluate microbiological culture rate and positivity of corneoscleral rim and cornea storage media as well as possible risk factors for contamination with real-world data.METHODS: Data of consecutive cornea donors implanted in the reference centre from January 2013 to January 2018 were reviewed. Information about cornea characteristics (donor demographic data, endothelial cell density, type of cornea conservation, days of storage, and precut vs full-thickness tissue), and microbiological culture information (corneoscleral rim vs storage sample, positive result) were statistically analysed.RESULTS: During the study period, 1369 corneas (737 donors) were implanted. Cultures were performed in 76.8% (n = 1052) of them and were positive in 3.2% of cases, mainly bacteria (84.4%). Corneas preserved in hypothermia represented 61.8% of all positive microbiology results (p < 0.001). Other analysed risk factors did not reach statistically significant association with microbiological positivity. None of the 34 cases with positive microbiological cultures reported ocular infection for the recipients in at least 6 months' follow-up.CONCLUSIONS: Microbiological tests rate in real-world practice are high despite not being compulsory. Organotypic cultured corneas showed a statistically less positivity in corneoscleral and storage medium than hypothermic ones, resulting in another advantage of this kind of cornea storage. Although precut corneas are thought to present less microbiological positivity, a statistically significant association was not found in the present study.
AB - OBJECTIVES: To evaluate microbiological culture rate and positivity of corneoscleral rim and cornea storage media as well as possible risk factors for contamination with real-world data.METHODS: Data of consecutive cornea donors implanted in the reference centre from January 2013 to January 2018 were reviewed. Information about cornea characteristics (donor demographic data, endothelial cell density, type of cornea conservation, days of storage, and precut vs full-thickness tissue), and microbiological culture information (corneoscleral rim vs storage sample, positive result) were statistically analysed.RESULTS: During the study period, 1369 corneas (737 donors) were implanted. Cultures were performed in 76.8% (n = 1052) of them and were positive in 3.2% of cases, mainly bacteria (84.4%). Corneas preserved in hypothermia represented 61.8% of all positive microbiology results (p < 0.001). Other analysed risk factors did not reach statistically significant association with microbiological positivity. None of the 34 cases with positive microbiological cultures reported ocular infection for the recipients in at least 6 months' follow-up.CONCLUSIONS: Microbiological tests rate in real-world practice are high despite not being compulsory. Organotypic cultured corneas showed a statistically less positivity in corneoscleral and storage medium than hypothermic ones, resulting in another advantage of this kind of cornea storage. Although precut corneas are thought to present less microbiological positivity, a statistically significant association was not found in the present study.
KW - Bacteria
KW - Cornea
KW - Corneal Transplantation
KW - Eye Banks
KW - Humans
KW - Organ Preservation
KW - Tissue Donors
UR - http://www.scopus.com/inward/record.url?scp=85100109193&partnerID=8YFLogxK
UR - https://www.mendeley.com/catalogue/321d7fde-41e5-3324-91d3-3bb4e6c95fee/
U2 - 10.1038/s41433-020-01342-8
DO - 10.1038/s41433-020-01342-8
M3 - Article
C2 - 33469128
SN - 0950-222X
JO - Eye
JF - Eye
ER -